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Int. J. Mol. Sci. 2013, 14(9), 18009-18023; doi:10.3390/ijms140918009

Celf1 Is Required for Formation of Endoderm-Derived Organs in Zebrafish

Gene Regulation Research, Nara Institute Science and Technology, 8916-5 Takayama, Nara 630-0101, Japan
* Author to whom correspondence should be addressed.
Received: 25 June 2013 / Revised: 21 August 2013 / Accepted: 26 August 2013 / Published: 3 September 2013
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We recently reported that an RNA binding protein called Cugbp Elav-like family member 1 (Celf1) regulates somite symmetry and left-right patterning in zebrafish. In this report, we show additional roles of Celf1 in zebrafish organogenesis. When celf1 is knocked down by using an antisense morpholino oligonucleotides (MO), liver buds fail to form, and pancreas buds do not form a cluster, suggesting earlier defects in endoderm organogenesis. As expected, we found failures in endoderm cell growth and migration during gastrulation in embryos injected with celf1-MOs. RNA immunoprecipitation revealed that Celf1 binds to gata5 and cdc42 mRNAs which are known to be involved in cell growth and migration, respectively. Our results therefore suggest that Celf1 regulates proper organogenesis of endoderm-derived tissues by regulating the expression of such targets.
Keywords: post-transcriptional regulation; endoderm; liver; pancreas; movement; proliferation post-transcriptional regulation; endoderm; liver; pancreas; movement; proliferation
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Tahara, N.; Bessho, Y.; Matsui, T. Celf1 Is Required for Formation of Endoderm-Derived Organs in Zebrafish. Int. J. Mol. Sci. 2013, 14, 18009-18023.

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