Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2013, 14(9), 18024-18040; doi:10.3390/ijms140918024
Article

Progressive Changes in Inflammatory and Matrix Adherence of Bronchial Epithelial Cells with Persistent Respiratory Syncytial Virus (RSV) Infection (Progressive Changes in RSV Infection)

1,2
, 1,* , 1
, 1
, 1
, 3
, 1
 and 1
Received: 7 July 2013; in revised form: 16 August 2013 / Accepted: 21 August 2013 / Published: 3 September 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [2327 KB, uploaded 19 June 2014]
Abstract: In addition to the acute manifestations of respiratory syncytial virus (RSV), persistent infection may be associated with long-term complications in the development of chronic respiratory diseases. To understand the mechanisms underlying RSV-induced long-term consequences, we established an in vitro RSV (strain A2) infection model using human bronchial epithelial (16HBE) cells that persists over four generations and analyzed cell inflammation and matrix adherence. Cells infected with RSV at multiplicity of infection (MOI) 0.0067 experienced cytolytic or abortive infections in the second generation (G2) or G3 but mostly survived up to G4. Cell morphology, leukocyte and matrix adherence of the cells did not change in G1 or G2, but subsequently, leukocyte adherence and cytokine/chemokine secretion, partially mediated by intercellular adhesion molecule-1 (ICAM-1), increased drastically, and matrix adherence, partially mediated by E-cadherin, decreased until the cells died. Tumor necrosis factor-α (TNF-α) secretion was inhibited by ICAM-1 antibody in infected-16HBE cells, suggesting that positive feedback between TNF-α secretion and ICAM-1 expression may be significant in exacerbated inflammation. These data demonstrate the susceptibility of 16HBE cells to RSV and their capacity to produce long-term progressive RSV infection, which may contribute to inflammation mobilization and epithelial shedding.
Keywords: respiratory syncytial virus; human bronchial epithelial cells; adherence; adhesive molecule; cytokine; chemokine respiratory syncytial virus; human bronchial epithelial cells; adherence; adhesive molecule; cytokine; chemokine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Liu, X.; Qin, X.; Xiang, Y.; Liu, H.; Gao, G.; Qin, L.; Liu, C.; Qu, X. Progressive Changes in Inflammatory and Matrix Adherence of Bronchial Epithelial Cells with Persistent Respiratory Syncytial Virus (RSV) Infection (Progressive Changes in RSV Infection). Int. J. Mol. Sci. 2013, 14, 18024-18040.

AMA Style

Liu X, Qin X, Xiang Y, Liu H, Gao G, Qin L, Liu C, Qu X. Progressive Changes in Inflammatory and Matrix Adherence of Bronchial Epithelial Cells with Persistent Respiratory Syncytial Virus (RSV) Infection (Progressive Changes in RSV Infection). International Journal of Molecular Sciences. 2013; 14(9):18024-18040.

Chicago/Turabian Style

Liu, Xiaoai; Qin, Xiaoqun; Xiang, Yang; Liu, Huijun; Gao, Ge; Qin, Ling; Liu, Chi; Qu, Xiangping. 2013. "Progressive Changes in Inflammatory and Matrix Adherence of Bronchial Epithelial Cells with Persistent Respiratory Syncytial Virus (RSV) Infection (Progressive Changes in RSV Infection)." Int. J. Mol. Sci. 14, no. 9: 18024-18040.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert