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The Characterisation of Pluripotent and Multipotent Stem Cells Using Fourier Transform Infrared Microspectroscopy
Department of Anatomy and Developmental Biology, Faculty of Medicine, Nursing and Health Sciences, Monash University, Wellington Road, Clayton, Victoria 3800, Australia
Centre for Biospectroscopy and the School of Chemistry, Monash University, Clayton, Victoria 3800, Australia
Murdoch Childrens Research Institute, the Royal Children's Hospital, Parkville, Victoria 3052, Australia
Australian Synchrotron, 800 Blackburn Road, Clayton, Victoria 3168, Australia
* Author to whom correspondence should be addressed.
Received: 29 May 2013; in revised form: 22 July 2013 / Accepted: 22 July 2013 / Published: 26 August 2013
Abstract: Fourier transform infrared (FTIR) microspectroscopy shows potential as a benign, objective and rapid tool to screen pluripotent and multipotent stem cells for clinical use. It offers a new experimental approach that provides a holistic measurement of macromolecular composition such that a signature representing the internal cellular phenotype is obtained. The use of this technique therefore contributes information that is complementary to that acquired by conventional genetic and immunohistochemical methods.
Keywords: Fourier transform infrared microspectroscopy; pluripotent stem cells; multipotent stem cells; regenerative medicine
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Cao, J.; Ng, E.S.; McNaughton, D.; Stanley, E.G.; Elefanty, A.G.; Tobin, M.J.; Heraud, P. The Characterisation of Pluripotent and Multipotent Stem Cells Using Fourier Transform Infrared Microspectroscopy. Int. J. Mol. Sci. 2013, 14, 17453-17476.
Cao J, Ng ES, McNaughton D, Stanley EG, Elefanty AG, Tobin MJ, Heraud P. The Characterisation of Pluripotent and Multipotent Stem Cells Using Fourier Transform Infrared Microspectroscopy. International Journal of Molecular Sciences. 2013; 14(9):17453-17476.
Cao, Julie; Ng, Elizabeth S.; McNaughton, Donald; Stanley, Edouard G.; Elefanty, Andrew G.; Tobin, Mark J.; Heraud, Philip. 2013. "The Characterisation of Pluripotent and Multipotent Stem Cells Using Fourier Transform Infrared Microspectroscopy." Int. J. Mol. Sci. 14, no. 9: 17453-17476.