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Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population
Laboratory of Genetics, Wageningen University, Wageningen, 6700 AH, The Netherlands
Department of Biotechnology, College of Agriculture, Bu-Ali Sina University, Hamedan 65174, Iran
Faculty of Agriculture, Department of Genetics, Cairo University, Giza, Gamaa St. 12613, Egypt
Natural Product Laboratory, Institute of Biology, Leiden University, Leiden, Sylviusweg 72, 2333BE, The Netherlands
Plant Breeding Institute, Christian Albrechts University, Kiel, Olshausenstrasse 40, D-24098, Germany
* Author to whom correspondence should be addressed.
Received: 27 May 2013; in revised form: 9 July 2013 / Accepted: 17 July 2013 / Published: 25 July 2013
Abstract: The genetic basis of the wide variation for nutritional traits in Brassica rapa is largely unknown. A new Recombinant Inbred Line (RIL) population was profiled using High Performance Liquid Chromatography (HPLC) and Nuclear Magnetic Resonance (NMR) analysis to detect quantitative trait loci (QTLs) controlling seed tocopherol and seedling metabolite concentrations. RIL population parent L58 had a higher level of glucosinolates and phenylpropanoids, whereas levels of sucrose, glucose and glutamate were higher in the other RIL population parent, R-o-18. QTL related to seed tocopherol (α-, β-, γ-, δ-, α-⁄γ- and total tocopherol) concentrations were detected on chromosomes A3, A6, A9 and A10, explaining 11%–35% of the respective variation. The locus on A3 co-locates with the BrVTE1gene, encoding tocopherol cyclase. NMR spectroscopy identified the presence of organic/amino acid, sugar/glucosinolate and aromatic compounds in seedlings. QTL positions were obtained for most of the identified compounds. Compared to previous studies, novel loci were found for glucosinolate concentrations. This work can be used to design markers for marker-assisted selection of nutritional compounds in B. rapa.
Keywords: Brassica rapa; secondary metabolites; nutritional value; Quantitative Trait Loci; tocopherol; glucosinolates
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Bagheri, H.; El-Soda, M.; Kim, H.K.; Fritsche, S.; Jung, C.; Aarts, M.G.M. Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population. Int. J. Mol. Sci. 2013, 14, 15561-15577.
Bagheri H, El-Soda M, Kim HK, Fritsche S, Jung C, Aarts MGM. Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population. International Journal of Molecular Sciences. 2013; 14(8):15561-15577.
Bagheri, Hedayat; El-Soda, Mohamed; Kim, Hye K.; Fritsche, Steffi; Jung, Christian; Aarts, Mark G.M. 2013. "Genetic Analysis of Health-Related Secondary Metabolites in a Brassica rapa Recombinant Inbred Line Population." Int. J. Mol. Sci. 14, no. 8: 15561-15577.