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Int. J. Mol. Sci. 2013, 14(8), 15578-15594; doi:10.3390/ijms140815578

Phyto-SERM Constitutes from Flemingia macrophylla

6,7,8,*  and 1,5,*
1 Graduate Institute of Natural Products, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 80708, Taiwan 2 Department of Pharmacognosy and Natural Products Chemistry, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street, Abassia, Cairo 11566, Egypt 3 Graduate Institute of Natural Products, Chang Gung University, Taoyuan 333, Taiwan 4 Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 110, Taiwan 5 Cancer Center, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan 6 School of Pharmacy, College of Pharmacy, China Medical University, Taichung 404, Taiwan 7 Chinese Medicine Research and Development Center, China Medical University Hospital, Taichung 404, Taiwan 8 Center for Molecular Medicine, China Medical University Hospital, Taichung 404, Taiwan
* Authors to whom correspondence should be addressed.
Received: 15 March 2013 / Revised: 6 July 2013 / Accepted: 15 July 2013 / Published: 26 July 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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The methanolic extract of Flemingia macrophylla roots exhibited significant estrogenic activity in the transgenic plant assay system which was comparable to the activity of soybean extract. Utilizing estrogenic activity-guided fractionation, one new compound, fleminigin, together with 23 known compounds were isolated from F. macrophylla roots’ methanolic extract. The structure of the new compound was identified based on intensive spectroscopic analysis and the full spectral data for one of the isolated compounds, flemichin E, was introduced for the first time in the current investigation. The estrogenic and anti-estrogenic activities of the isolated compounds were evaluated revealing that the isolated isoflavonoids may act as partial estrogen agonists, as well as antagonists. Additionally, the anti-inflammatory and the cytotoxic activities of the isolated compounds were studied. These results suggested the potential applications of F. macrophylla extract and its isolated compounds as selective estrogen receptor modulators (SERMs).
Keywords: Flemingia macrophylla; menopausal; phytoestrogen; fleminigin; flemichin E; pER8:GUS Flemingia macrophylla; menopausal; phytoestrogen; fleminigin; flemichin E; pER8:GUS
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Lai, W.-C.; Tsui, Y.-T.; Singab, A.N.B.; El-Shazly, M.; Du, Y.-C.; Hwang, T.-L.; Wu, C.-C.; Yen, M.-H.; Lee, C.-K.; Hou, M.-F.; Wu, Y.-C.; Chang, F.-R. Phyto-SERM Constitutes from Flemingia macrophylla. Int. J. Mol. Sci. 2013, 14, 15578-15594.

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