Int. J. Mol. Sci. 2013, 14(8), 15330-15347; doi:10.3390/ijms140815330
Article

Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat

1 State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing 100101, China 2 Department of Life Science, Tangshan Normal University, 156 North Jianshe Road, Tangshan 063000, China
* Author to whom correspondence should be addressed.
Received: 7 June 2013; in revised form: 11 July 2013 / Accepted: 15 July 2013 / Published: 24 July 2013
(This article belongs to the Special Issue Advances in Molecular Plant Biology)
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Abstract: Hexaploid wheat displays limited genetic variation. As a direct A and B genome donor of hexaploid wheat, tetraploid wheat represents an important gene pool for cultivated bread wheat. Many disease resistant genes express conserved domains of the nucleotide-binding site and leucine-rich repeats (NBS-LRR). In this study, we isolated a CC-NBS-LRR gene locating on chromosome 7B from durum wheat variety Italy 363, and designated it TdRGA-7Ba. Its open reading frame was 4014 bp, encoding a 1337 amino acid protein with a complete NBS domain and 18 LRR repeats, sharing 44.7% identity with the PM3B protein. TdRGA-7Ba expression was continuously seen at low levels and was highest in leaves. TdRGA-7Ba has another allele TdRGA-7Bb with a 4 bp deletion at position +1892 in other cultivars of tetraploid wheat. In Ae. speltoides, as a B genome progenitor, both TdRGA-7Ba and TdRGA-7Bb were detected. In all six species of hexaploid wheats (AABBDD), only TdRGA-7Bb existed. Phylogenic analysis showed that all TdRGA-7Bb type genes were grouped in one sub-branch. We speculate that TdRGA-7Bb was derived from a TdRGA-7Ba mutation, and it happened in Ae. speltoides. Both types of TdRGA-7B participated in tetraploid wheat formation. However, only the TdRGA-7Bb was retained in hexaploid wheat.
Keywords: wheat; NBS-LRR gene; gene mutation; molecular evolution

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MDPI and ACS Style

Gong, C.; Cao, S.; Fan, R.; Wei, B.; Chen, G.; Wang, X.; Li, Y.; Zhang, X. Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat. Int. J. Mol. Sci. 2013, 14, 15330-15347.

AMA Style

Gong C, Cao S, Fan R, Wei B, Chen G, Wang X, Li Y, Zhang X. Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat. International Journal of Molecular Sciences. 2013; 14(8):15330-15347.

Chicago/Turabian Style

Gong, Caiyan; Cao, Shuanghe; Fan, Renchun; Wei, Bo; Chen, Guiping; Wang, Xianping; Li, Yiwen; Zhang, Xiangqi. 2013. "Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat." Int. J. Mol. Sci. 14, no. 8: 15330-15347.

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