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Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, No.1 West Beichen Road, Chaoyang District, Beijing 100101, China
Department of Life Science, Tangshan Normal University, 156 North Jianshe Road, Tangshan 063000, China
* Author to whom correspondence should be addressed.
Received: 7 June 2013; in revised form: 11 July 2013 / Accepted: 15 July 2013 / Published: 24 July 2013
Abstract: Hexaploid wheat displays limited genetic variation. As a direct A and B genome donor of hexaploid wheat, tetraploid wheat represents an important gene pool for cultivated bread wheat. Many disease resistant genes express conserved domains of the nucleotide-binding site and leucine-rich repeats (NBS-LRR). In this study, we isolated a CC-NBS-LRR gene locating on chromosome 7B from durum wheat variety Italy 363, and designated it TdRGA-7Ba. Its open reading frame was 4014 bp, encoding a 1337 amino acid protein with a complete NBS domain and 18 LRR repeats, sharing 44.7% identity with the PM3B protein. TdRGA-7Ba expression was continuously seen at low levels and was highest in leaves. TdRGA-7Ba has another allele TdRGA-7Bb with a 4 bp deletion at position +1892 in other cultivars of tetraploid wheat. In Ae. speltoides, as a B genome progenitor, both TdRGA-7Ba and TdRGA-7Bb were detected. In all six species of hexaploid wheats (AABBDD), only TdRGA-7Bb existed. Phylogenic analysis showed that all TdRGA-7Bb type genes were grouped in one sub-branch. We speculate that TdRGA-7Bb was derived from a TdRGA-7Ba mutation, and it happened in Ae. speltoides. Both types of TdRGA-7B participated in tetraploid wheat formation. However, only the TdRGA-7Bb was retained in hexaploid wheat.
Keywords: wheat; NBS-LRR gene; gene mutation; molecular evolution
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Gong, C.; Cao, S.; Fan, R.; Wei, B.; Chen, G.; Wang, X.; Li, Y.; Zhang, X. Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat. Int. J. Mol. Sci. 2013, 14, 15330-15347.
Gong C, Cao S, Fan R, Wei B, Chen G, Wang X, Li Y, Zhang X. Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat. International Journal of Molecular Sciences. 2013; 14(8):15330-15347.
Gong, Caiyan; Cao, Shuanghe; Fan, Renchun; Wei, Bo; Chen, Guiping; Wang, Xianping; Li, Yiwen; Zhang, Xiangqi. 2013. "Identification and Phylogenetic Analysis of a CC-NBS-LRR Encoding Gene Assigned on Chromosome 7B of Wheat." Int. J. Mol. Sci. 14, no. 8: 15330-15347.