Next Article in Journal
Serum Oxidant and Antioxidant Status Following an All-Out 21-km Run in Adolescent Runners Undergoing Professional Training—A One-Year Prospective Trial
Previous Article in Journal
Disease-Causing Mutations in BEST1 Gene Are Associated with Altered Sorting of Bestrophin-1 Protein
Previous Article in Special Issue
Alleviation of Osmotic Stress Effects by Exogenous Application of Salicylic or Abscisic Acid on Wheat Seedlings
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2013, 14(7), 15141-15166; doi:10.3390/ijms140715141

Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level

1
DSMZ–German Collection for Microorganisms and Cell Cultures, Inhoffenstr. 7b, 38124 Braunschweig, Germany
2
Centraalbureau voor Schimmelcultures–Fungal Biodiversity Center, Uppsalalaan 8, 3584CT Utrecht, The Netherlands
*
Author to whom correspondence should be addressed.
Received: 18 April 2013 / Revised: 19 May 2013 / Accepted: 23 May 2013 / Published: 22 July 2013
(This article belongs to the Special Issue Abiotic and Biotic Stress Tolerance Mechanisms in Plants)
View Full-Text   |   Download PDF [1565 KB, uploaded 19 June 2014]   |  

Abstract

Biotechnological approaches using genetic modifications such as homologous gene overexpression can be used to decode gene functions under well-defined circumstances. However, only the recording of the resulting phenotypes allows inferences about the impact of the modification on the organisms’ evolutionary, ecological or economic performance. We here compare a potato wild-type cell line with two genetically engineered cell cultures homologously overexpressing Pathogenesis Related Protein 10a (pr-10a). A detailed analysis of the relative gene-expression patterns of pr-10a and its regulators sebf and pti4 over time provides insights into the molecular response of heterotrophic cells to distinct osmotic and salt-stress conditions. Furthermore, this system serves as an exemplar for the tracing of respiration kinetics as a faster and more sensitive alternative to the laborious and time-consuming recording of growth curves. The utility and characteristics of the resulting data type and the requirements for its appropriate analysis are figured out. It is demonstrated how this novel type of phenotypic information together with the gene-expression-data provides valuable insights into the effect of genetic modifications on the behaviour of cells on both the molecular and the macroscopic level.
Keywords: pathogenesis related protein 10a; cell-respiration; TTC; Solanum tuberosum cv. Désirée pathogenesis related protein 10a; cell-respiration; TTC; Solanum tuberosum cv. Désirée
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Vaas, L.A.I.; Marheine, M.; Sikorski, J.; Göker, M.; Schumacher, H.-M. Impacts of pr-10a Overexpression at the Molecular and the Phenotypic Level. Int. J. Mol. Sci. 2013, 14, 15141-15166.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top