Int. J. Mol. Sci. 2013, 14(7), 14996-15016; doi:10.3390/ijms140714996
Article

Induced Production of 1-Methoxy-indol-3-ylmethyl Glucosinolate by Jasmonic Acid and Methyl Jasmonate in Sprouts and Leaves of Pak Choi (Brassica rapa ssp. chinensis)

1 Department of Quality Research, Leibniz-Institute of Vegetable and Ornamental Crops Grossbeeren and Erfurt e.V., Theodor-Echtermeyer-Weg 1, 14979 Grossbeeren, Germany 2 Department of Nutritional Toxicology, German Institute of Human Nutrition Potsdam-Rehbr├╝cke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany
* Author to whom correspondence should be addressed.
Received: 9 May 2013; in revised form: 13 June 2013 / Accepted: 24 June 2013 / Published: 18 July 2013
(This article belongs to the Special Issue Molecular Research in Plant Secondary Metabolism)
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Abstract: Pak choi plants (Brassica rapa ssp. chinensis) were treated with different signaling molecules methyl jasmonate, jasmonic acid, linolenic acid, and methyl salicylate and were analyzed for specific changes in their glucosinolate profile. Glucosinolate levels were quantified using HPLC-DAD-UV, with focus on induction of indole glucosinolates and special emphasis on 1-methoxy-indol-3-ylmethyl glucosinolate. Furthermore, the effects of the different signaling molecules on indole glucosinolate accumulation were analyzed on the level of gene expression using semi-quantitative realtime RT-PCR of selected genes. The treatments with signaling molecules were performed on sprouts and mature leaves to determine ontogenetic differences in glucosinolate accumulation and related gene expression. The highest increase of indole glucosinolate levels, with considerable enhancement of the 1-methoxy-indol-3-ylmethyl glucosinolate content, was achieved with treatments of sprouts and mature leaves with methyl jasmonate and jasmonic acid. This increase was accompanied by increased expression of genes putatively involved in the indole glucosinolate biosynthetic pathway. The high levels of indole glucosinolates enabled the plant to preferentially produce the respective breakdown products after tissue damage. Thus, pak choi plants treated with methyl jasmonate or jasmonic acid, are a valuable tool to analyze the specific protection functions of 1-methoxy-indole-3-carbinole in the plants defense strategy in the future.
Keywords: Brassica rapa ssp. chinensis; signaling molecules; methyl jasmonate; 1-methoxy-indol-3-ylmethyl glucosinolate; glucosinolate biosynthesis pathway; indole glucosinolates; gene expression

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MDPI and ACS Style

Wiesner, M.; Hanschen, F.S.; Schreiner, M.; Glatt, H.; Zrenner, R. Induced Production of 1-Methoxy-indol-3-ylmethyl Glucosinolate by Jasmonic Acid and Methyl Jasmonate in Sprouts and Leaves of Pak Choi (Brassica rapa ssp. chinensis). Int. J. Mol. Sci. 2013, 14, 14996-15016.

AMA Style

Wiesner M, Hanschen FS, Schreiner M, Glatt H, Zrenner R. Induced Production of 1-Methoxy-indol-3-ylmethyl Glucosinolate by Jasmonic Acid and Methyl Jasmonate in Sprouts and Leaves of Pak Choi (Brassica rapa ssp. chinensis). International Journal of Molecular Sciences. 2013; 14(7):14996-15016.

Chicago/Turabian Style

Wiesner, Melanie; Hanschen, Franziska S.; Schreiner, Monika; Glatt, Hansruedi; Zrenner, Rita. 2013. "Induced Production of 1-Methoxy-indol-3-ylmethyl Glucosinolate by Jasmonic Acid and Methyl Jasmonate in Sprouts and Leaves of Pak Choi (Brassica rapa ssp. chinensis)." Int. J. Mol. Sci. 14, no. 7: 14996-15016.

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