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Int. J. Mol. Sci. 2013, 14(6), 12313-12328; doi:10.3390/ijms140612313
Article

Contribution of the Tyr-1 in Plantaricin149a to Disrupt Phospholipid Model Membranes

1
, 2
, 2
, 3
 and 1,*
Received: 21 December 2012; in revised form: 7 May 2013 / Accepted: 25 May 2013 / Published: 7 June 2013
(This article belongs to the Special Issue Phospholipids: Molecular Sciences 2012)
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Abstract: Plantaricin149a (Pln149a) is a cationic antimicrobial peptide, which was suggested to cause membrane destabilization via the carpet mechanism. The mode of action proposed to this antimicrobial peptide describes the induction of an amphipathic α-helix from Ala7 to Lys20, while the N-terminus residues remain in a coil conformation after binding. To better investigate this assumption, the purpose of this study was to determine the contributions of the Tyr1 in Pln149a in the binding to model membranes to promote its destabilization. The Tyr to Ser substitution increased the dissociation constant (KD) of the antimicrobial peptide from the liposomes (approximately three-fold higher), and decreased the enthalpy of binding to anionic vesicles from −17.2 kcal/mol to −10.2 kcal/mol. The peptide adsorption/incorporation into the negatively charged lipid vesicles was less effective with the Tyr1 substitution and peptide Pln149a perturbed the liposome integrity more than the analog, Pln149S. Taken together, the peptide-lipid interactions that govern the Pln149a antimicrobial activity are found not only in the amphipathic helix, but also in the N-terminus residues, which take part in enthalpic contributions due to the allocation at a lipid-aqueous interface.
Keywords: antimicrobial peptide; membrane models; peptide-lipid interaction; plantaricin antimicrobial peptide; membrane models; peptide-lipid interaction; plantaricin
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lopes, J.L.S.; Gómara, M.J.; Haro, I.; Tonarelli, G.; Beltramini, L.M. Contribution of the Tyr-1 in Plantaricin149a to Disrupt Phospholipid Model Membranes. Int. J. Mol. Sci. 2013, 14, 12313-12328.

AMA Style

Lopes JLS, Gómara MJ, Haro I, Tonarelli G, Beltramini LM. Contribution of the Tyr-1 in Plantaricin149a to Disrupt Phospholipid Model Membranes. International Journal of Molecular Sciences. 2013; 14(6):12313-12328.

Chicago/Turabian Style

Lopes, José L.S.; Gómara, Maria J.; Haro, Isabel; Tonarelli, Georgina; Beltramini, Leila M. 2013. "Contribution of the Tyr-1 in Plantaricin149a to Disrupt Phospholipid Model Membranes." Int. J. Mol. Sci. 14, no. 6: 12313-12328.


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