Int. J. Mol. Sci. 2013, 14(5), 9722-9736; doi:10.3390/ijms14059722
Article

Molecular Interaction of a New Antibacterial Polymer with a Supported Lipid Bilayer Measured by an in situ Label-Free Optical Technique

Received: 19 March 2013; in revised form: 21 April 2013 / Accepted: 2 May 2013 / Published: 6 May 2013
(This article belongs to the Special Issue Antimicrobial Polymers)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: The interaction of the antibacterial polymer–branched poly(ethylene imine) substituted with quaternary ammonium groups, PEO and alkyl chains, PEI25QI5J5A815–with a solid supported lipid bilayer was investigated using surface sensitive optical waveguide spectroscopy. The analysis of the optogeometrical parameters was extended developing a new composite layer model in which the structural and optical anisotropy of the molecular layers was taken into consideration. Following in situ the change of optical birefringence we were able to determine the composition of the lipid/polymer surface layer as well as the displacement of lipid bilayer by the antibacterial polymer without using additional labeling. Comparative assessment of the data of layer thickness and optical anisotropy helps to reveal the molecular mechanism of antibacterial effect of the polymer investigated.
Keywords: antibacterial polymer; supported lipid bilayer; optical waveguide spectroscopy; optical anisotropy; label-free measurement
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MDPI and ACS Style

Horvath, R.; Kobzi, B.; Keul, H.; Moeller, M.; Kiss, É. Molecular Interaction of a New Antibacterial Polymer with a Supported Lipid Bilayer Measured by an in situ Label-Free Optical Technique. Int. J. Mol. Sci. 2013, 14, 9722-9736.

AMA Style

Horvath R, Kobzi B, Keul H, Moeller M, Kiss É. Molecular Interaction of a New Antibacterial Polymer with a Supported Lipid Bilayer Measured by an in situ Label-Free Optical Technique. International Journal of Molecular Sciences. 2013; 14(5):9722-9736.

Chicago/Turabian Style

Horvath, Robert; Kobzi, Balázs; Keul, Helmut; Moeller, Martin; Kiss, Éva. 2013. "Molecular Interaction of a New Antibacterial Polymer with a Supported Lipid Bilayer Measured by an in situ Label-Free Optical Technique." Int. J. Mol. Sci. 14, no. 5: 9722-9736.

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