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IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength
Department of Neurology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China
Department of Biomedicine, China National Center for Biotechnology Development, Beijing 100036, China
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 18 February 2013; in revised form: 28 March 2013 / Accepted: 15 April 2013 / Published: 25 April 2013
Abstract: Botulinum toxin type-A (Btx-A), a powerful therapeutic tool in various medical specialties, requires repeated injections to maintain its effect. Therefore, novel methods to prolong the effective duration time of Btx-A are highly needed. Rats were assigned to three major groups: control group (n = 30), Btx-A group (n = 30), and IGF-1 Ab groups. IGF-1 Ab groups were composed by sub-groups A1–A5 (each has 25 rats) for the subsequent IGF-1Ab dose-effect study. Muscle strength was determined by a survey system for rat lower limbs nerve and muscle function. Muscle-specific receptor tyrosine kinase (MuSK), Insulin-like growth factor binding protein-5 (IGFBP5), and growth-associated protein, 43-kDa (GAP43) were determined by real-time polymerase chain reactions (PCRs) and Western blot. We found that Btx-A decreased the muscle strength, with a paralysis maintained for 70 days. IGF-1Ab prolonged the effective duration time of Btx-A. Real-time PCRs and Western blot showed that IGF-1Ab delayed the increase of MuSK and IGFBP5 after Btx-A injection, without affecting GAP43. These results indicate that IGF-1Ab might prolong the effective duration time of Btx-A on muscle strength through delaying the increase of MuSK. It would be interesting to determine whether IGF-1Ab can be used as an auxiliary measure to the Btx-A treatment in the future.
Keywords: botulinum toxin; insulin-like growth factor 1; muscle strength; muscle-specific receptor tyrosine kinase
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MDPI and ACS Style
Jin, L.; Pan, L.; Liu, W.; Guo, Y.; Zheng, Y.; Guan, Q.; Nie, Z. IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength. Int. J. Mol. Sci. 2013, 14, 9051-9061.
Jin L, Pan L, Liu W, Guo Y, Zheng Y, Guan Q, Nie Z. IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength. International Journal of Molecular Sciences. 2013; 14(5):9051-9061.
Jin, Lingjing; Pan, Lizhen; Liu, Wuchao; Guo, Yan; Zheng, Yuguo; Guan, Qiang; Nie, Zhiyu. 2013. "IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength." Int. J. Mol. Sci. 14, no. 5: 9051-9061.