Abstract: Botulinum toxin type-A (Btx-A), a powerful therapeutic tool in various medical specialties, requires repeated injections to maintain its effect. Therefore, novel methods to prolong the effective duration time of Btx-A are highly needed. Rats were assigned to three major groups: control group (n = 30), Btx-A group (n = 30), and IGF-1 Ab groups. IGF-1 Ab groups were composed by sub-groups A1–A5 (each has 25 rats) for the subsequent IGF-1Ab dose-effect study. Muscle strength was determined by a survey system for rat lower limbs nerve and muscle function. Muscle-specific receptor tyrosine kinase (MuSK), Insulin-like growth factor binding protein-5 (IGFBP5), and growth-associated protein, 43-kDa (GAP43) were determined by real-time polymerase chain reactions (PCRs) and Western blot. We found that Btx-A decreased the muscle strength, with a paralysis maintained for 70 days. IGF-1Ab prolonged the effective duration time of Btx-A. Real-time PCRs and Western blot showed that IGF-1Ab delayed the increase of MuSK and IGFBP5 after Btx-A injection, without affecting GAP43. These results indicate that IGF-1Ab might prolong the effective duration time of Btx-A on muscle strength through delaying the increase of MuSK. It would be interesting to determine whether IGF-1Ab can be used as an auxiliary measure to the Btx-A treatment in the future.
Keywords: botulinum toxin; insulin-like growth factor 1; muscle strength; muscle-specific receptor tyrosine kinase
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Jin, L.; Pan, L.; Liu, W.; Guo, Y.; Zheng, Y.; Guan, Q.; Nie, Z. IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength. Int. J. Mol. Sci. 2013, 14, 9051-9061.
Jin L, Pan L, Liu W, Guo Y, Zheng Y, Guan Q, Nie Z. IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength. International Journal of Molecular Sciences. 2013; 14(5):9051-9061.
Jin, Lingjing; Pan, Lizhen; Liu, Wuchao; Guo, Yan; Zheng, Yuguo; Guan, Qiang; Nie, Zhiyu. 2013. "IGF-1 Antibody Prolongs the Effective Duration Time of Botulinum Toxin in Decreasing Muscle Strength." Int. J. Mol. Sci. 14, no. 5: 9051-9061.