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Int. J. Mol. Sci. 2013, 14(5), 10369-10382; doi:10.3390/ijms140510369
Article

The Role of Peritoneal Alternatively Activated Macrophages in the Process of Peritoneal Fibrosis Related to Peritoneal Dialysis

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Received: 4 March 2013; in revised form: 7 May 2013 / Accepted: 8 May 2013 / Published: 17 May 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: It has been confirmed that alternatively activated macrophages (M2) participate in tissue remodeling and fibrosis occurrence, but the effect of M2 on peritoneal fibrosis related to peritoneal dialysis (PD) hasn’t been elucidated. This study was therefore conducted to assess the association between M2 and peritoneal fibrosis related to PD. In this study, peritoneal fibrosis was induced by intraperitoneal (i.p.) injection of Lactate-4.25% dialysate (100 mL/kg) to C57BL/6J mice for 28 days, and liposome-encapsulated clodronate (LC, the specific scavenger of macrophages) was used to treat the peritoneal fibrosis mice model by i.p. injection at day 18 and day 21. All animals were sacrificed at day 29. Parietal peritonea were stained with Masson’s trichrome, and the expression of type I collagen (Col-I), fibronectin, mannose receptor (CD206), transforming growth factor beta (TGF-β), chemokine receptor 7 (CCR7), chitinase 3-like 3 (Ym-1) and arginase-1 (Arg-1) was determined by Western blotting, immunofluorescence and quantitative real-time PCR. Our results revealed that peritoneal thickness, Col-I, fibronectin, CD206, TGF-β, Ym-1 and Arg-1 were upregulated in the peritoneal fibrosis mice model, and all of these indexes were downregulated in those treated with LC. Additionally, there was no difference in the level of CCR7 between the model and treatment group. Our study indicated that peritoneal M2 played an important role in the process of peritoneal fibrosis related to PD and might be a potential target for intervention therapy of peritoneal fibrosis.
Keywords: peritoneal dialysis; peritoneal fibrosis related to PD; alternatively activated macrophages; liposome-encapsulated clodronate peritoneal dialysis; peritoneal fibrosis related to PD; alternatively activated macrophages; liposome-encapsulated clodronate
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, J.; Jiang, Z.-P.; Su, N.; Fan, J.-J.; Ruan, Y.-P.; Peng, W.-X.; Li, Y.-F.; Yu, X.-Q. The Role of Peritoneal Alternatively Activated Macrophages in the Process of Peritoneal Fibrosis Related to Peritoneal Dialysis. Int. J. Mol. Sci. 2013, 14, 10369-10382.

AMA Style

Wang J, Jiang Z-P, Su N, Fan J-J, Ruan Y-P, Peng W-X, Li Y-F, Yu X-Q. The Role of Peritoneal Alternatively Activated Macrophages in the Process of Peritoneal Fibrosis Related to Peritoneal Dialysis. International Journal of Molecular Sciences. 2013; 14(5):10369-10382.

Chicago/Turabian Style

Wang, Jie; Jiang, Zong-Pei; Su, Ning; Fan, Jin-Jin; Ruan, Yi-Ping; Peng, Wen-Xing; Li, Ya-Fang; Yu, Xue-Qing. 2013. "The Role of Peritoneal Alternatively Activated Macrophages in the Process of Peritoneal Fibrosis Related to Peritoneal Dialysis." Int. J. Mol. Sci. 14, no. 5: 10369-10382.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert