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Int. J. Mol. Sci. 2013, 14(4), 8625-8637; doi:10.3390/ijms14048625
Technical Note

Single Nucleotide Polymorphisms in the Leptin-a Gene and Associations with Growth Traits in the Orange-Spotted Grouper (Epinephelus coioides)

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Received: 7 February 2013; in revised form: 11 April 2013 / Accepted: 16 April 2013 / Published: 22 April 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Leptin is a multifunctional protein involved in processes such as body weight regulation, energy expenditure, fat metabolism, food intake, and appetite regulation. Duplicate leptin genes, leptin-a and leptin-b, were previously detected in the orange-spotted grouper. In this study, we cloned the full-length open reading frame (ORF) of the leptin-a gene in the orange-spotted grouper, searched for polymorphisms, and performed association analyses between these polymorphisms and seven growth traits. Six polymorphisms, consisting of 2 SNPs in intron 1 (c.182T > G, c.183G > T) and 4 SNPs in exon 2 (c.339C > G, c.345C > T, c.447G > A, c.531C > T), were identified and genotyped in 200 individuals. The c.182T > G and c.183G > T polymorphisms showed complete linkage and were analyzed together. Association analyses revealed that the c.182 + 183TG > GT polymorphism was significantly associated with body weight (BWT) and body width (BWH), with the AB (TG/GT) genotype showing positive effects on growth traits. Additionally, the SNP c.447G > A was significantly associated with BWT, BWH, overall length (OL), trunk width (TW), and head length (HL), with the GA genotype displaying positive effects on growth traits. The c.531C > T SNP showed a close association between the TT genotype and decreased growth. Our results demonstrate that several polymorphisms in the leptin-a gene are associated with growth traits and can be used for marker-assisted selection (MAS) in orange-spotted grouper populations.
Keywords: leptin; single nucleotide polymorphisms (SNPs); molecular markers; orange-spotted grouper (Epinephelus coioides); growth traits; association analysis leptin; single nucleotide polymorphisms (SNPs); molecular markers; orange-spotted grouper (Epinephelus coioides); growth traits; association analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wei, Y.; Huang, H.; Meng, Z.; Zhang, Y.; Luo, J.; Chen, G.; Lin, H. Single Nucleotide Polymorphisms in the Leptin-a Gene and Associations with Growth Traits in the Orange-Spotted Grouper (Epinephelus coioides). Int. J. Mol. Sci. 2013, 14, 8625-8637.

AMA Style

Wei Y, Huang H, Meng Z, Zhang Y, Luo J, Chen G, Lin H. Single Nucleotide Polymorphisms in the Leptin-a Gene and Associations with Growth Traits in the Orange-Spotted Grouper (Epinephelus coioides). International Journal of Molecular Sciences. 2013; 14(4):8625-8637.

Chicago/Turabian Style

Wei, Yun; Huang, Hai; Meng, Zining; Zhang, Yong; Luo, Jian; Chen, Guohua; Lin, Haoran. 2013. "Single Nucleotide Polymorphisms in the Leptin-a Gene and Associations with Growth Traits in the Orange-Spotted Grouper (Epinephelus coioides)." Int. J. Mol. Sci. 14, no. 4: 8625-8637.



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