A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs)
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Design of the Sandwich Immunoassay for Salmonella Detection
2.3 Functionalization of MNPs with Antibody
2.3.1. Synthesis of QDs-Ab Conjugates
2.3.2. Salmonella Sample Analysis
3. Results and Discussion
3.1. Characterization of QD-Ab
3.2. Optimization of the Immunoassay System
3.3. Performance Assessment of Salmonella Detection
4. Conclusions
Acknowledgments
References
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Kuang, H.; Cui, G.; Chen, X.; Yin, H.; Yong, Q.; Xu, L.; Peng, C.; Wang, L.; Xu, C. A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs). Int. J. Mol. Sci. 2013, 14, 8603-8610. https://doi.org/10.3390/ijms14048603
Kuang H, Cui G, Chen X, Yin H, Yong Q, Xu L, Peng C, Wang L, Xu C. A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs). International Journal of Molecular Sciences. 2013; 14(4):8603-8610. https://doi.org/10.3390/ijms14048603
Chicago/Turabian StyleKuang, Hua, Gang Cui, Xiujin Chen, Honghong Yin, Qianqian Yong, Liguang Xu, Chifang Peng, Libing Wang, and Chuanlai Xu. 2013. "A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs)" International Journal of Molecular Sciences 14, no. 4: 8603-8610. https://doi.org/10.3390/ijms14048603