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Int. J. Mol. Sci. 2013, 14(5), 9111-9125; doi:10.3390/ijms14059111
Article

Tumour Cell Labelling by Magnetic Nanoparticles with Determination of Intracellular Iron Content and Spatial Distribution of the Intracellular Iron

*  and *
Received: 1 March 2013; in revised form: 26 March 2013 / Accepted: 2 April 2013 / Published: 26 April 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
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Abstract: Magnetically labelled cells are used for in vivo cell tracking by MRI, used for the clinical translation of cell-base therapies. Studies involving magnetic labelled cells may include separation of labelled cells, targeted delivery and controlled release of drugs, contrast enhanced MRI and magnetic hyperthermia for the in situ ablation of tumours. Dextran-coated super-paramagnetic iron oxide (SPIO) ferumoxides are used clinically as an MR contrast agents primarily for hepatic imaging. The material is also widely used for in vitro cell labelling, as are other SPIO-based particles. Our results on the uptake by human cancer cell lines of ferumoxides indicate that electroporation in the presence of protamine sulphate (PS) results in rapid high uptake of SPIO nanoparticles (SPIONs) by parenchymal tumour cells without significant impairment of cell viability. Quantitative determination of cellular iron uptake performed by colorimetric assay is in agreement with data from the literature. These results on intracellular iron content together with the intracellular distribution of SPIONs by magnetic force microscopy (MFM) following in vitro uptake by parenchymal tumour cells confirm the potential of this technique for clinical tumour cell detection and destruction.
Keywords: magnetic nanoparticles; superparamagnetic; SPIONs; nanomedicine; magnetic cell labelling; magnetic force microscopy magnetic nanoparticles; superparamagnetic; SPIONs; nanomedicine; magnetic cell labelling; magnetic force microscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Z.; Cuschieri, A. Tumour Cell Labelling by Magnetic Nanoparticles with Determination of Intracellular Iron Content and Spatial Distribution of the Intracellular Iron. Int. J. Mol. Sci. 2013, 14, 9111-9125.

AMA Style

Wang Z, Cuschieri A. Tumour Cell Labelling by Magnetic Nanoparticles with Determination of Intracellular Iron Content and Spatial Distribution of the Intracellular Iron. International Journal of Molecular Sciences. 2013; 14(5):9111-9125.

Chicago/Turabian Style

Wang, Zhigang; Cuschieri, Alfred. 2013. "Tumour Cell Labelling by Magnetic Nanoparticles with Determination of Intracellular Iron Content and Spatial Distribution of the Intracellular Iron." Int. J. Mol. Sci. 14, no. 5: 9111-9125.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert