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Int. J. Mol. Sci. 2013, 14(4), 8603-8610; doi:10.3390/ijms14048603
Article

A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs)

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Received: 6 February 2013; in revised form: 15 April 2013 / Accepted: 16 April 2013 / Published: 22 April 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
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Abstract: Simple immuno-magnetic separation tandem fluorescent probes based on quantum dots-antibody (QDs-Ab) were developed to detect Salmonella with sensitivity of 500 cfu mL−1. With two monoclonal antibodies, which recognize different antigenic determinant on the surface of Salmonella, we prepared antibody-coated magnetic nanoparticles (MNPs) and conjugates of QDs-Ab. The immune-magnetic beads were verified with high enrichment efficiency for Salmonella (90%). A sandwich structure formed if the Salmonella solution was mixed together with immune-beads and QDs-Ab, and the fluorescent single from QDs was related to the amount of Salmonella. A linear response between fluorescence intensity and various concentrations of Salmonella (2.5 × 103 to 1.95 × 108 cfu mL−1) were observed with this proposed method. The total assay time for Salmonella was 30 min, and no cross-reaction to other microbial strains, such as Staphylococcus aureus, Escherichia coli (E. coli) and Escherichia coli O157:H7 (E. coli O157:H7), were found using this detection system. All our results showed that the simple homogeneous immunoassay could be applied in Salmonella screening without time-consuming extra-enrichment of bacteria.
Keywords: Salmonella; fluorescent immunoassay; magnetic separation; quantum dots Salmonella; fluorescent immunoassay; magnetic separation; quantum dots
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kuang, H.; Cui, G.; Chen, X.; Yin, H.; Yong, Q.; Xu, L.; Peng, C.; Wang, L.; Xu, C. A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs). Int. J. Mol. Sci. 2013, 14, 8603-8610.

AMA Style

Kuang H, Cui G, Chen X, Yin H, Yong Q, Xu L, Peng C, Wang L, Xu C. A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs). International Journal of Molecular Sciences. 2013; 14(4):8603-8610.

Chicago/Turabian Style

Kuang, Hua; Cui, Gang; Chen, Xiujin; Yin, Honghong; Yong, Qianqian; Xu, Liguang; Peng, Chifang; Wang, Libing; Xu, Chuanlai. 2013. "A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs)." Int. J. Mol. Sci. 14, no. 4: 8603-8610.



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