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Int. J. Mol. Sci. 2013, 14(4), 8603-8610; doi:10.3390/ijms14048603

A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs)

School of Food Science and Technology, State Key Lab of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
YanCheng Teachers University, Yancheng 214122, Jiangsu, China
Research Center of Hunan Entry-Exit Inspection and Quarantine Bureau, Changsha 410001, Hunan, China
Author to whom correspondence should be addressed.
Received: 6 February 2013 / Revised: 15 April 2013 / Accepted: 16 April 2013 / Published: 22 April 2013
(This article belongs to the Special Issue Magnetic Nanoparticles 2013)
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Simple immuno-magnetic separation tandem fluorescent probes based on quantum dots-antibody (QDs-Ab) were developed to detect Salmonella with sensitivity of 500 cfu mL−1. With two monoclonal antibodies, which recognize different antigenic determinant on the surface of Salmonella, we prepared antibody-coated magnetic nanoparticles (MNPs) and conjugates of QDs-Ab. The immune-magnetic beads were verified with high enrichment efficiency for Salmonella (90%). A sandwich structure formed if the Salmonella solution was mixed together with immune-beads and QDs-Ab, and the fluorescent single from QDs was related to the amount of Salmonella. A linear response between fluorescence intensity and various concentrations of Salmonella (2.5 × 103 to 1.95 × 108 cfu mL−1) were observed with this proposed method. The total assay time for Salmonella was 30 min, and no cross-reaction to other microbial strains, such as Staphylococcus aureus, Escherichia coli (E. coli) and Escherichia coli O157:H7 (E. coli O157:H7), were found using this detection system. All our results showed that the simple homogeneous immunoassay could be applied in Salmonella screening without time-consuming extra-enrichment of bacteria. View Full-Text
Keywords: Salmonella; fluorescent immunoassay; magnetic separation; quantum dots Salmonella; fluorescent immunoassay; magnetic separation; quantum dots

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Kuang, H.; Cui, G.; Chen, X.; Yin, H.; Yong, Q.; Xu, L.; Peng, C.; Wang, L.; Xu, C. A One-Step Homogeneous Sandwich Immunosensor for Salmonella Detection Based on Magnetic Nanoparticles (MNPs) and Quantum Dots (QDs). Int. J. Mol. Sci. 2013, 14, 8603-8610.

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