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Int. J. Mol. Sci. 2013, 14(4), 7959-7978; doi:10.3390/ijms14047959
Article

Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines

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Received: 28 February 2013; in revised form: 21 March 2013 / Accepted: 1 April 2013 / Published: 11 April 2013
(This article belongs to the Special Issue Advances in Cancer Diagnosis)
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Abstract: Receptor for Advanced Glycation End Products (RAGE) is an oncogenic trans-membranous receptor overexpressed in various human cancers. However, the role of RAGE in breast cancer development and proliferation is still unclear. In this study, we demonstrated that RAGE expression levels are correlated to the degree of severity of breast cancer. Furthermore, there is a decrease in the proliferation of all sub-types of breast cancer, MCF-7, SK-Br-3 and MDA-MB-231, as a result of the effect of RAGE siRNA. RAGE siRNA arrested cells in the G1 phase and inhibited DNA synthesis (p < 0.05). Moreover, qRT-PCR and Western Blot results demonstrated that RAGE siRNA decreases the expression of transcriptional factor NF-κB p65 as well as the expression of cell proliferation markers PCNA and cyclinD1. RAGE and RAGE ligands can thus be considered as possible targets for breast cancer management and therapy.
Keywords: siRNA; proliferation; breast cancer siRNA; proliferation; breast cancer
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Radia, A.-M.; Yaser, A.-M.; Ma, X.; Zhang, J.; Yang, C.; Dong, Q.; Rong, P.; Ye, B.; Liu, S.; Wang, W. Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines. Int. J. Mol. Sci. 2013, 14, 7959-7978.

AMA Style

Radia A-M, Yaser A-M, Ma X, Zhang J, Yang C, Dong Q, Rong P, Ye B, Liu S, Wang W. Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines. International Journal of Molecular Sciences. 2013; 14(4):7959-7978.

Chicago/Turabian Style

Radia, AL-Madhagi; Yaser, AL-Madhagi; Ma, Xiaoqian; Zhang, Juan; Yang, Cejun; Dong, Qiong; Rong, Pengfei; Ye, Bin; Liu, Sheng; Wang, Wei. 2013. "Specific siRNA Targeting Receptor for Advanced Glycation End Products (RAGE) Decreases Proliferation in Human Breast Cancer Cell Lines." Int. J. Mol. Sci. 14, no. 4: 7959-7978.


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