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Int. J. Mol. Sci. 2013, 14(4), 8358-8366; doi:10.3390/ijms14048358
Short Note

Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells

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Received: 25 February 2013; in revised form: 3 April 2013 / Accepted: 8 April 2013 / Published: 16 April 2013
(This article belongs to the Special Issue Advances in Cancer Diagnosis)
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Abstract: Methods of fluorescence spectroscopy and microscopy—including intensity and lifetime (FLIM) images—are used to examine uptake, intracellular location and interaction of the chemotherapeutic drug doxorubicin in MCF-7 human breast cancer cells as a function of cholesterol content. By comparing cells with natural and decreased cholesterol levels after 2 h or 24 h incubation with doxorubicin, we observed that higher fluorescence intensities and possibly shortened fluorescence lifetimes—reflecting increased uptake of the drug and more pronounced drug response—are concomitant with higher membrane fluidity.
Keywords: cancer cells; doxorubicin; cholesterol; fluorescence lifetime imaging microscopy (FLIM) cancer cells; doxorubicin; cholesterol; fluorescence lifetime imaging microscopy (FLIM)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Weber, P.; Wagner, M.; Schneckenburger, H. Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells. Int. J. Mol. Sci. 2013, 14, 8358-8366.

AMA Style

Weber P, Wagner M, Schneckenburger H. Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells. International Journal of Molecular Sciences. 2013; 14(4):8358-8366.

Chicago/Turabian Style

Weber, Petra; Wagner, Michael; Schneckenburger, Herbert. 2013. "Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells." Int. J. Mol. Sci. 14, no. 4: 8358-8366.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert