Int. J. Mol. Sci. 2013, 14(4), 8358-8366; doi:10.3390/ijms14048358
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Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells

Institut für Angewandte Forschung, Hochschule Aalen, Anton-Huber Str. 21, 73430 Aalen, Germany
* Author to whom correspondence should be addressed.
Received: 25 February 2013; in revised form: 3 April 2013 / Accepted: 8 April 2013 / Published: 16 April 2013
(This article belongs to the Special Issue Advances in Cancer Diagnosis)
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Abstract: Methods of fluorescence spectroscopy and microscopy—including intensity and lifetime (FLIM) images—are used to examine uptake, intracellular location and interaction of the chemotherapeutic drug doxorubicin in MCF-7 human breast cancer cells as a function of cholesterol content. By comparing cells with natural and decreased cholesterol levels after 2 h or 24 h incubation with doxorubicin, we observed that higher fluorescence intensities and possibly shortened fluorescence lifetimes—reflecting increased uptake of the drug and more pronounced drug response—are concomitant with higher membrane fluidity.
Keywords: cancer cells; doxorubicin; cholesterol; fluorescence lifetime imaging microscopy (FLIM)

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MDPI and ACS Style

Weber, P.; Wagner, M.; Schneckenburger, H. Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells. Int. J. Mol. Sci. 2013, 14, 8358-8366.

AMA Style

Weber P, Wagner M, Schneckenburger H. Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells. International Journal of Molecular Sciences. 2013; 14(4):8358-8366.

Chicago/Turabian Style

Weber, Petra; Wagner, Michael; Schneckenburger, Herbert. 2013. "Cholesterol Dependent Uptake and Interaction of Doxorubicin in MCF-7 Breast Cancer Cells." Int. J. Mol. Sci. 14, no. 4: 8358-8366.

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