Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Int. J. Mol. Sci. 2013, 14(3), 5998-6011; doi:10.3390/ijms14035998
Article

Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions

1
, 2
, 2
, 3
 and 2,*
Received: 18 January 2013; in revised form: 3 March 2013 / Accepted: 5 March 2013 / Published: 15 March 2013
(This article belongs to the Special Issue Molecular Research in Urology)
View Full-Text   |   Download PDF [4329 KB, uploaded 19 June 2014]
Abstract: Given that podocalyxin (PCX) and nestin play important roles in podocyte morphogenesis and the maintenance of structural integrity, we examined whether the expression and localization of these two podocyte proteins were influenced in the early stage of various hemodynamic conditions. Mice kidney tissues were prepared by in vivo cryotechnique (IVCT). The distribution of glomeruli and podocyte proteins was visualized with DAB staining, confocal laser scanning microscopy and immunoelectron microscopy. The mRNA levels were examined by real-time quantitative PCR. The results showed the following: Under the normal condition, PCX stained intensely along glomerular epithelial cells, whereas nestin was clearly staining in the endothelial cells and appeared only weakly in the podocytes. Under the acute hypertensive and cardiac arrest conditions, PCX and nestin staining was not clear, with a disarranged distribution, but the colocalization of PCX and nestin was apparent under this condition. In addition, under the acute hypertensive and cardiac arrest conditions, the mRNA levels of PCX and nestin were significantly decreased. Collectively, the abnormal redistribution and decreased mRNA expressions of PCX and nestin are important molecular events at the early stage of podocyte injury during hemodynamic disorders. IVCT may have more advantages for morphological analysis when researching renal diseases.
Keywords: podocalyxin; nestin; acute hypertension; cardiac arrest; in vivo cryotechnique podocalyxin; nestin; acute hypertension; cardiac arrest; in vivo cryotechnique
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Li, K.; Wang, J.; Yin, X.; Zhai, X.; Li, Z. Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions. Int. J. Mol. Sci. 2013, 14, 5998-6011.

AMA Style

Li K, Wang J, Yin X, Zhai X, Li Z. Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions. International Journal of Molecular Sciences. 2013; 14(3):5998-6011.

Chicago/Turabian Style

Li, Kai; Wang, Juan; Yin, Xiaohui; Zhai, Xiaoyue; Li, Zilong. 2013. "Alteration of Podocyte Protein Expression and Localization in the Early Stage of Various Hemodynamic Conditions." Int. J. Mol. Sci. 14, no. 3: 5998-6011.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert