5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)
Abstract
:1. Introduction
2. Results
Diagnosis | Aneusomy | Ploidy | ||||
---|---|---|---|---|---|---|
Monosomy * | Trisomy ** | Total | Triploidy | Tetraploidy | Total | |
Cervical Epithelium: | ||||||
Control | 2 | 2 | 4 | 1 | - | 1 |
LG-SIL | 3 | 4 | 7 | 1 | 1 | 2 |
HG-SIL | 3 | 5 | 8 | 3 | 6 | 9 |
Lymphocytes: | ||||||
Control | - | - | - | - | - | - |
LG-SIL | - | 2 | 2 | - | 1 | 1 |
HG-SIL | 3 | 2 | 5 | - | 2 | 2 |
Tissue Type | Control (n = 10) | LG-SIL (n = 10) | HG-SIL (n = 10) | Kruskal Wallis | p | |||
---|---|---|---|---|---|---|---|---|
Median | Mean Rank | Median | Mean Rank | Median | Mean Rank | |||
Cervical Epitelium: | ||||||||
ID-WG | 1.14 × 108 | 5.80 | 6.12 × 109 | 15.70 | 4.69 × 109 | 25.00 | 23.79 | 0.000 |
Total NC | 4600 | 13.25 | 4600 | 14.70 | 4600 | 18.55 | 3.525 | 0.172 |
ID Chromosome-1 | 1.65 × 104 | 8.20 | 3.01 × 104 | 15.50 | 8.68 × 104 | 22.80 | 13.75 | 0.001 |
NC-1 | 200 | 14.50 | 200 | 14.70 | 200 | 17.30 | 1.04 | 0.594 |
Lymphocytes: | ||||||||
ID-WG | 3.58 × 104 | 5.70 | 20.79 × 104 | 15.90 | 165.99 × 104 | 24.90 | 23.81 | 0.000 |
Total NC | 4600 | 14.50 | 4600 | 15.95 | 4600 | 16.05 | 1.04 | 0.595 |
ID Chromosome-1 | 1.19 × 104 | 5.50 | 9.89 × 104 | 18.10 | 25.88 × 104 | 22.90 | 20.84 | 0.000 |
NC-1 | 200 | 15.00 | 200 | 16.45 | 200 | 15.05 | 0.645 | 0.724 |
HPV GENOTYPING | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diagnosis | 16 | 18 | 31 | 33 | 39 | 44 | 51 | 52 | 54 | 58 | 68 | 70 | 82 |
LSIL | 4 | 1 | 4 | 3 | 2 | 4 | 4 | 6 | 4 | 1 | - | - | 2 |
HSIL | 6 | 2 | 2 | - | 4 | 2 | 4 | 3 | 3 | 1 | 1 | 1 | 2 |
TOTAL | 10 | 3 | 6 | 3 | 6 | 6 | 8 | 9 | 7 | 2 | 1 | 1 | 4 |
3. Experimental Section
3.1. Study Population
3.2. Detection and Genotyping of HPV
3.3. Cell Preparation
3.4. Slide Preparation
3.5. DBD-FISH
3.6. Image Analysis
3.7. Chromosome-Orientation Fluorescence in Situ Hybridization (CO-FISH)
3.8. Statistical Analysis
4. Discussion
5. Conclusions
Acknowledgments
Conflict of Interest
References
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Cortés-Gutiérrez, E.I.; Ortíz-Hernández, B.L.; Dávila-Rodríguez, M.I.; Cerda-Flores, R.M.; Fernández, J.L.; López-Fernández, C.; Gosálvez, J. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH). Int. J. Mol. Sci. 2013, 14, 4135-4147. https://doi.org/10.3390/ijms14024135
Cortés-Gutiérrez EI, Ortíz-Hernández BL, Dávila-Rodríguez MI, Cerda-Flores RM, Fernández JL, López-Fernández C, Gosálvez J. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH). International Journal of Molecular Sciences. 2013; 14(2):4135-4147. https://doi.org/10.3390/ijms14024135
Chicago/Turabian StyleCortés-Gutiérrez, Elva I., Brenda L. Ortíz-Hernández, Martha I. Dávila-Rodríguez, Ricardo M. Cerda-Flores, José Luis Fernández, Carmen López-Fernández, and Jaime Gosálvez. 2013. "5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)" International Journal of Molecular Sciences 14, no. 2: 4135-4147. https://doi.org/10.3390/ijms14024135