Int. J. Mol. Sci. 2013, 14(2), 3467-3486; doi:10.3390/ijms14023467
Article

Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker

Silvia Borrego 1,2 email, Antonio Vazquez 3 email, Francisco Dasí 4 email, Concha Cerdá 1,2 email, Antonio Iradi 5 email, Carmen Tormos 6 email, Julia M. Sánchez 6 email, Leticia Bagán 6 email, Javier Boix 7 email, Cristóbal Zaragoza 3 email, Jordi Camps 8 email and Guillermo Sáez 1,2,6,* email
1 Clinical Analysis Service, CDB-General University Hospital, Avda. Tres Cruces s/n., Valencia 46014, Spain 2 Oxidative Stress Research Group, SEQC, Calle Padilla 323, Barcelona 08025, Spain 3 Department of General Surgery and Digestive Diseases, General University Hospital, Avda. Tres Cruces s/n, Valencia 46014, Spain 4 Valencia Clinical Hospital Research Foundation (INCLIVA), Avda. Blasco Ibáñez 15, Valencia 46010, Spain 5 Department of Physiology, Faculty of Medicine, University of Valencia, Avda. Blasco Ibañez 15, Valencia 46010, Spain 6 Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Valencia, INCLIVA. Avda. Blasco Ibañez 15, Valencia 46010, Spain 7 Department of Pathology, Faculty of Medicine, University of Valencia, Avda. Blasco Ibañez 15, Valencia 46010, Spain 8 Bimedical Research Center, Sant Joan University Hospital, Health Institute of Research Pere Virgili, Rovira i Virigili University, Reus 43201, Spain
* Author to whom correspondence should be addressed.
Received: 6 October 2012; in revised form: 8 January 2013 / Accepted: 11 January 2013 / Published: 6 February 2013
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases)
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Abstract: We characterized the oxidative stress (OS) status by the levels of reduced/oxidized glutathione (GSH/GSSG), malondialdehyde (MDA) and the mutagenic base 8-oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) in human gastric carcinoma (HGC) samples and compared the results with normal tissue from the same patients. We also analyzed 8-oxo-dG in peripheral mononuclear cells (PMNC) and urine from healthy control subjects and in affected patients in the basal state and one, three, six, nine and twelve months after tumor resection. The levels of DNA repair enzyme mRNA expression (hOGG1, RAD51, MUYTH and MTH1) were determined in tumor specimens and compared with normal mucosa. Tumor specimens exhibited increased levels of MDA and 8-oxo-dG compared with normal gastric tissue. GSH levels were also increased, while GSSG levels remained stable. DNA repair enzyme mRNA expression was induced in the tumor tissues. Levels of 8-oxo-dG were significantly elevated in both urine and PMNC of gastric cancer patients compared with healthy controls. After gastrectomy, the levels of the damaged base in urine and PMNC decreased progressively to values close to those found in the healthy population. The high levels of 8-oxo-dG in urine may be related to the increased induction of DNA repair activity in tumor tissue, and the changes observed after tumor resection support its potential use as a tumor marker.
Keywords: 8-oxo-dG; DNA repair enzymes; gastric cancer; oxidative stress; tumor marker

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Cite This Article

MDPI and ACS Style

Borrego, S.; Vazquez, A.; Dasí, F.; Cerdá, C.; Iradi, A.; Tormos, C.; Sánchez, J.M.; Bagán, L.; Boix, J.; Zaragoza, C.; Camps, J.; Sáez, G. Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker. Int. J. Mol. Sci. 2013, 14, 3467-3486.

AMA Style

Borrego S, Vazquez A, Dasí F, Cerdá C, Iradi A, Tormos C, Sánchez JM, Bagán L, Boix J, Zaragoza C, Camps J, Sáez G. Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker. International Journal of Molecular Sciences. 2013; 14(2):3467-3486.

Chicago/Turabian Style

Borrego, Silvia; Vazquez, Antonio; Dasí, Francisco; Cerdá, Concha; Iradi, Antonio; Tormos, Carmen; Sánchez, Julia M.; Bagán, Leticia; Boix, Javier; Zaragoza, Cristóbal; Camps, Jordi; Sáez, Guillermo. 2013. "Oxidative Stress and DNA Damage in Human Gastric Carcinoma: 8-Oxo-7'8-dihydro-2'-deoxyguanosine (8-oxo-dG) as a Possible Tumor Marker." Int. J. Mol. Sci. 14, no. 2: 3467-3486.

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