Int. J. Mol. Sci. 2013, 14(2), 4135-4147; doi:10.3390/ijms14024135
Article

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

1 Department of Genetics, Northeastern Biomedical Research Center, The Mexican Social Security Institute (IMSS), 64720 Monterrey, Mexico 2 Nursing Faculty, Autonomous University of Nuevo León, 64460 Monterrey, Mexico 3 Section of Genetics and Research Unit, Hospital Teresa Herrera, Juan Canalejo University Hospital Complex, 15006 La Coruña, Spain 4 Unit of Genetics, Department of Biology, Autonomous University of Madrid, 20849 Madrid, Spain
* Author to whom correspondence should be addressed.
Received: 24 December 2012; in revised form: 28 January 2013 / Accepted: 28 January 2013 / Published: 19 February 2013
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases)
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Abstract: We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.
Keywords: cervical neoplasia; DBD-FISH; 5-bp classical satellite DNA

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MDPI and ACS Style

Cortés-Gutiérrez, E.I.; Ortíz-Hernández, B.L.; Dávila-Rodríguez, M.I.; Cerda-Flores, R.M.; Fernández, J.L.; López-Fernández, C.; Gosálvez, J. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH). Int. J. Mol. Sci. 2013, 14, 4135-4147.

AMA Style

Cortés-Gutiérrez EI, Ortíz-Hernández BL, Dávila-Rodríguez MI, Cerda-Flores RM, Fernández JL, López-Fernández C, Gosálvez J. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH). International Journal of Molecular Sciences. 2013; 14(2):4135-4147.

Chicago/Turabian Style

Cortés-Gutiérrez, Elva I.; Ortíz-Hernández, Brenda L.; Dávila-Rodríguez, Martha I.; Cerda-Flores, Ricardo M.; Fernández, José L.; López-Fernández, Carmen; Gosálvez, Jaime. 2013. "5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)." Int. J. Mol. Sci. 14, no. 2: 4135-4147.

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