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Int. J. Mol. Sci. 2013, 14(2), 4135-4147; doi:10.3390/ijms14024135

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

1
Department of Genetics, Northeastern Biomedical Research Center, The Mexican Social Security Institute (IMSS), 64720 Monterrey, Mexico
2
Nursing Faculty, Autonomous University of Nuevo León, 64460 Monterrey, Mexico
3
Section of Genetics and Research Unit, Hospital Teresa Herrera, Juan Canalejo University Hospital Complex, 15006 La Coruña, Spain
4
Unit of Genetics, Department of Biology, Autonomous University of Madrid, 20849 Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 24 December 2012 / Revised: 28 January 2013 / Accepted: 28 January 2013 / Published: 19 February 2013
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases)
View Full-Text   |   Download PDF [1372 KB, 19 June 2014; original version 19 June 2014]   |  

Abstract

We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1. View Full-Text
Keywords: cervical neoplasia; DBD-FISH; 5-bp classical satellite DNA cervical neoplasia; DBD-FISH; 5-bp classical satellite DNA
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Cortés-Gutiérrez, E.I.; Ortíz-Hernández, B.L.; Dávila-Rodríguez, M.I.; Cerda-Flores, R.M.; Fernández, J.L.; López-Fernández, C.; Gosálvez, J. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH). Int. J. Mol. Sci. 2013, 14, 4135-4147.

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