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Int. J. Mol. Sci. 2013, 14(2), 4135-4147; doi:10.3390/ijms14024135
Article

5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH)

1,* , 1, 1, 2
, 3
, 4 and 4
1 Department of Genetics, Northeastern Biomedical Research Center, The Mexican Social Security Institute (IMSS), 64720 Monterrey, Mexico 2 Nursing Faculty, Autonomous University of Nuevo León, 64460 Monterrey, Mexico 3 Section of Genetics and Research Unit, Hospital Teresa Herrera, Juan Canalejo University Hospital Complex, 15006 La Coruña, Spain 4 Unit of Genetics, Department of Biology, Autonomous University of Madrid, 20849 Madrid, Spain
* Author to whom correspondence should be addressed.
Received: 24 December 2012 / Revised: 28 January 2013 / Accepted: 28 January 2013 / Published: 19 February 2013
(This article belongs to the Special Issue DNA Damage and Repair in Degenerative Diseases)
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Abstract

We aimed to evaluate the association between the progressive stages of cervical neoplasia and DNA damage in 5-bp classical satellite DNA sequences from chromosome-1 in cervical epithelium and in peripheral blood lymphocytes using DNA breakage detection/fluorescence in situ hybridization (DBD-FISH). A hospital-based unmatched case-control study was conducted in 2011 with a sample of 30 women grouped according to disease stage and selected according to histological diagnosis; 10 with low-grade squamous intraepithelial lesions (LG-SIL), 10 with high-grade SIL (HG-SIL), and 10 with no cervical lesions, from the Unidad Medica de Alta Especialidad of The Mexican Social Security Institute, IMSS, Mexico. Specific chromosome damage levels in 5-bp classical satellite DNA sequences from chromosome-1 were evaluated in cervical epithelium and peripheral blood lymphocytes using the DBD-FISH technique. Whole-genome DNA hybridization was used as a reference for the level of damage. Results of Kruskal-Wallis test showed a significant increase according to neoplastic development in both tissues. The instability of 5-bp classical satellite DNA sequences from chromosome-1 was evidenced using chromosome-orientation FISH. In conclusion, we suggest that the progression to malignant transformation involves an increase in the instability of 5-bp classical satellite DNA sequences from chromosome-1.
Keywords: cervical neoplasia; DBD-FISH; 5-bp classical satellite DNA cervical neoplasia; DBD-FISH; 5-bp classical satellite DNA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cortés-Gutiérrez, E.I.; Ortíz-Hernández, B.L.; Dávila-Rodríguez, M.I.; Cerda-Flores, R.M.; Fernández, J.L.; López-Fernández, C.; Gosálvez, J. 5-bp Classical Satellite DNA Loci from Chromosome-1 Instability in Cervical Neoplasia Detected by DNA Breakage Detection/Fluorescence in Situ Hybridization (DBD-FISH). Int. J. Mol. Sci. 2013, 14, 4135-4147.

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