Abstract: A rapid sensitive and selective liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for determination of timosaponin AIII (TA-III) in rat plasma, using ginsenoside Re as an internal standard (IS). TA-III and the IS were detected in MRM mode with a negative ionization electrospray mass spectrometer. The calibration curves were linear over the concentration ranges from 11.14 to 1114 ng/mL and the lower limit of quantification (LLOQ) was 11.14 ng/mL. Intra-day and inter-day precisions (RSD) were within 10%, and accuracy ranged from 6.4% to 9.1%. The extraction recovery at three concentrations ranged from 92.3% to 95.5%. The validated method was successfully applied to monitor the concentrations of TA-III in rat plasma after intragastric administration. The best fit pharmacokinetic model to estimate the pharmacokinetic parameters was a single compartment model with weight of 1/x2 for oral administration groups of rats for TA-III.
Keywords: timosaponin AIII; LC-MS/MS; rat plasma; pharmacokinetic study
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Liu, Y.; Pu, Y.; Zhang, T.; Ding, Y.; Wang, B.; Cai, Z. Rapid and Sensitive Determination of Timosaponin AIII in Rat Plasma by LC-MS/MS and Its Pharmacokinetic Application. Int. J. Mol. Sci. 2013, 14, 3656-3670.
Liu Y, Pu Y, Zhang T, Ding Y, Wang B, Cai Z. Rapid and Sensitive Determination of Timosaponin AIII in Rat Plasma by LC-MS/MS and Its Pharmacokinetic Application. International Journal of Molecular Sciences. 2013; 14(2):3656-3670.
Liu, Yanping; Pu, Yiqiong; Zhang, Tong; Ding, Yue; Wang, Bing; Cai, Zhenzhen. 2013. "Rapid and Sensitive Determination of Timosaponin AIII in Rat Plasma by LC-MS/MS and Its Pharmacokinetic Application." Int. J. Mol. Sci. 14, no. 2: 3656-3670.