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Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds
Engineering Research Center for Plant Biotechnology and Germplasm Utilization, Ministry of Education, State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China
Wuhan Institute of Biotechnology, Biolake, Wuhan 430075, China
Healthgen Biotechnology Ltd. Co., Biolake, Wuhan 430075, China
* Author to whom correspondence should be addressed.
Received: 29 November 2012; in revised form: 22 January 2013 / Accepted: 31 January 2013 / Published: 7 February 2013
Abstract: Basic fibroblast growth factor (FGF-2) is an important member of the FGF gene family. It is widely used in clinical applications for scald and wound healing in order to stimulate cell proliferation. Further it is applied for inhibiting stem cell differentiation in cultures. Due to a shortage of plasma and low expression levels of recombinant rbFGF in conventional gene expression systems, we explored the production of recombinant rbFGF in rice grains (Oryza sativa bFGF, OsrbFGF). An expression level of up to 185.66 mg/kg in brown rice was obtained. A simple purification protocol was established with final recovery of 4.49% and resulting in a yield of OsrbFGF reaching up to 8.33 mg/kg OsrbFGF. The functional assay of OsrbFGF indicated that the stimulating cell proliferation activity on NIH/3T3 was the same as with commercialized rbFGF. Wound healing in vivo of OsrbFGF is equivalent to commercialized rbFGF. Our results indicate that rice endosperm is capable of expressing small molecular mass proteins, such as bFGF. This again demonstrates that rice endosperm is a promising system to express various biopharmaceutical proteins.
Keywords: recombinant human bFGF; rice endosperm; protein expression and processing; cell proliferation and wound healing
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MDPI and ACS Style
An, N.; Ou, J.; Jiang, D.; Zhang, L.; Liu, J.; Fu, K.; Dai, Y.; Yang, D. Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds. Int. J. Mol. Sci. 2013, 14, 3556-3567.
An N, Ou J, Jiang D, Zhang L, Liu J, Fu K, Dai Y, Yang D. Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds. International Journal of Molecular Sciences. 2013; 14(2):3556-3567.
An, Na; Ou, Jiquan; Jiang, Daiming; Zhang, Liping; Liu, Jingru; Fu, Kai; Dai, Ying; Yang, Daichang. 2013. "Expression of a Functional Recombinant Human Basic Fibroblast Growth Factor from Transgenic Rice Seeds." Int. J. Mol. Sci. 14, no. 2: 3556-3567.