Int. J. Mol. Sci. 2013, 14(2), 4349-4360; doi:10.3390/ijms14024349
Article

PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells

1email, 1email, 2email, 2email, 3email, 4email and 1,4,* email
Received: 16 December 2012; in revised form: 16 January 2013 / Accepted: 6 February 2013 / Published: 21 February 2013
(This article belongs to the Special Issue Plant-Derived Pharmaceuticals by Molecular Farming 2012)
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Abstract: In an earlier study, we isolated potamin-1 (PT-1), a 5.6-kDa trypsin-chymotrypsin protease inhibitor, from the tubers of a potato strain (Solanum tuberosum L cv. Gogu Valley). We established that PT-1 strongly inhibits pathogenic microbial strains, but not human bacterial strains, and that its sequence shows 62% homology with a serine protease inhibitor. In the present study, we isolated an antifungal and antibacterial peptide with no cytotoxicity from tubers of the same potato strain. The peptide (peptide-G2, PG-2) was isolated using salt-extraction, ultrafiltration and reverse-phase high performance liquid chromatography (RP-HPLC). Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) showed the protein to have a molecular mass of 3228.5 Da, while automated Edman degradation showed the N-terminal sequence of PG-2 to be LVKDNPLDISPKQVQALCTDLVIRCMCCC-. PG-2 exhibited antimicrobial activity against Candida albicans, a human pathogenic yeast strain, and Clavibacter michiganensis subsp. michiganensis, a plant late blight strain. PG-2 also showed antibacterial activity against Staphylococcus aureus, but did not lyse human red blood cells and was thermostable. Overall, these results suggest PG-2 may be a good candidate to serve as a natural antimicrobial agent, agricultural pesticide and/or food additive.
Keywords: peptide-G2; RP-HPLC; N-terminal sequence; human pathogenic; antimicrobial agent
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kim, J.-Y.; Gopal, R.; Kim, S.Y.; Seo, C.H.; Lee, H.B.; Cheong, H.; Park, Y. PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells. Int. J. Mol. Sci. 2013, 14, 4349-4360.

AMA Style

Kim J-Y, Gopal R, Kim SY, Seo CH, Lee HB, Cheong H, Park Y. PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells. International Journal of Molecular Sciences. 2013; 14(2):4349-4360.

Chicago/Turabian Style

Kim, Jin-Young; Gopal, Ramamourthy; Kim, Sang Y.; Seo, Chang H.; Lee, Hyang B.; Cheong, Hyeonsook; Park, Yoonkyung. 2013. "PG-2, a Potent AMP against Pathogenic Microbial Strains, from Potato (Solanum tuberosum L cv. Gogu Valley) Tubers Not Cytotoxic against Human Cells." Int. J. Mol. Sci. 14, no. 2: 4349-4360.


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