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Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles
Department of Bioengineering, Tatung University, 40 Zhongshan N. Rd. Sec. 3, Taipei 10452, Taiwan
* Author to whom correspondence should be addressed.
Received: 9 October 2013; in revised form: 2 December 2013 / Accepted: 3 December 2013 / Published: 11 December 2013
Abstract: Biodiesel, a non-toxic and biodegradable fuel, has recently become a major source of renewable alternative fuels. Utilization of lipase as a biocatalyst to produce biodiesel has advantages over common alkaline catalysts such as mild reaction conditions, easy product separation, and use of waste cooking oil as raw material. In this study, Pseudomonas cepacia lipase immobilized onto magnetic nanoparticles (MNP) was used for biodiesel production from waste cooking oil. The optimal dosage of lipase-bound MNP was 40% (w/w of oil) and there was little difference between stepwise addition of methanol at 12 h- and 24 h-intervals. Reaction temperature, substrate molar ratio (methanol/oil), and water content (w/w of oil) were optimized using response surface methodology (RSM). The optimal reaction conditions were 44.2 °C, substrate molar ratio of 5.2, and water content of 12.5%. The predicted and experimental molar conversions of fatty acid methyl esters (FAME) were 80% and 79%, respectively.
Keywords: biodiesel; lipase; magnetic nanoparticles; response surface methodology; waste cooking oil
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Yu, C.-Y.; Huang, L.-Y.; Kuan, I.-C.; Lee, S.-L. Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles. Int. J. Mol. Sci. 2013, 14, 24074-24086.
Yu C-Y, Huang L-Y, Kuan I-C, Lee S-L. Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles. International Journal of Molecular Sciences. 2013; 14(12):24074-24086.
Yu, Chi-Yang; Huang, Liang-Yu; Kuan, I-Ching; Lee, Shiow-Ling. 2013. "Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles." Int. J. Mol. Sci. 14, no. 12: 24074-24086.