Next Article in Journal
MMP-7 and fcDNA Serum Levels in Early NSCLC and Idiopathic Interstitial Pneumonia: Preliminary Study
Next Article in Special Issue
The Antidiabetic Drug Metformin Inhibits the Proliferation of Bladder Cancer Cells in Vitro and in Vivo
Previous Article in Journal
Optimized Production of Biodiesel from Waste Cooking Oil by Lipase Immobilized on Magnetic Nanoparticles
Previous Article in Special Issue
Prognostic Discrimination Using a 70-Gene Signature among Patients with Estrogen Receptor-Positive Breast Cancer and an Intermediate 21-Gene Recurrence Score
Int. J. Mol. Sci. 2013, 14(12), 24087-24096; doi:10.3390/ijms141224087

Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells

, 1,2,3
, 1,2,3
, 1,2,3
, 1,2,3
, 4
, 1,2,3
, 5
, 5
, 1,2,3
 and 1,2,3,*
1 Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China 2 Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China 3 First Clinical Department of Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Tianjin 300192, China 4 Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, Tianjin 300134, China 5 National Institute of Radiological Sciences, Chiba 263-8555, Japan
* Author to whom correspondence should be addressed.
Received: 8 October 2013 / Revised: 19 November 2013 / Accepted: 2 December 2013 / Published: 11 December 2013
(This article belongs to the Special Issue Molecular Bases of Cancer Research)
View Full-Text   |   Download PDF [756 KB, uploaded 19 June 2014]   |   Browse Figures


The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of radiation on the expression of X-linked inhibitor of apoptosis protein (XIAP) were detected by western blotting. The radiation-sensitising effects of ANTP-SmacN7 fusion proteins were observed by a clonogenic assay. The effects of drugs and radiation on tumour cell apoptosis were determined using Annexin V/FITC double staining. Changes in caspase-8, caspase-9 and caspase-3 were detected by western blot before and after ANTP-SmacN7 inhibition of XIAP. The ANTP-SmacN7 fusion protein could enter and accumulate in cells; in vitro XIAP expression of radiation-induced tumour cells was negatively correlated with tumour radiosensitivity. The ANTP-SmacN7 fusion protein promoted tumour cell apoptosis through the activation of caspase3. ANTP-SmacN7 fusion protein may reduce tumour cell radioresistance by inducing caspase3 activation.
Keywords: ANTP-smacN7 fusion protein; apoptosis; radiosensitivity ANTP-smacN7 fusion protein; apoptosis; radiosensitivity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |

MDPI and ACS Style

Du, L.Q.; Wang, Y.; Xu, C.; Cao, J.; Wang, Q.; Zhao, H.; Fan, F.Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells. Int. J. Mol. Sci. 2013, 14, 24087-24096.

View more citation formats

Related Articles

Article Metrics


Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert