Abstract: The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of radiation on the expression of X-linked inhibitor of apoptosis protein (XIAP) were detected by western blotting. The radiation-sensitising effects of ANTP-SmacN7 fusion proteins were observed by a clonogenic assay. The effects of drugs and radiation on tumour cell apoptosis were determined using Annexin V/FITC double staining. Changes in caspase-8, caspase-9 and caspase-3 were detected by western blot before and after ANTP-SmacN7 inhibition of XIAP. The ANTP-SmacN7 fusion protein could enter and accumulate in cells; in vitro XIAP expression of radiation-induced tumour cells was negatively correlated with tumour radiosensitivity. The ANTP-SmacN7 fusion protein promoted tumour cell apoptosis through the activation of caspase3. ANTP-SmacN7 fusion protein may reduce tumour cell radioresistance by inducing caspase3 activation.
Keywords: ANTP-smacN7 fusion protein; apoptosis; radiosensitivity
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Du, L.Q.; Wang, Y.; Xu, C.; Cao, J.; Wang, Q.; Zhao, H.; Fan, F.Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells. Int. J. Mol. Sci. 2013, 14, 24087-24096.
Du LQ, Wang Y, Xu C, Cao J, Wang Q, Zhao H, Fan FY, Wang B, Katsube T, Fan SJ, Liu Q. Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells. International Journal of Molecular Sciences. 2013; 14(12):24087-24096.
Du, Li Q.; Wang, Yan; Xu, Chang; Cao, Jia; Wang, Qin; Zhao, Hui; Fan, Fei Y.; Wang, Bing; Katsube, Takanori; Fan, Sai J.; Liu, Qiang. 2013. "Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells." Int. J. Mol. Sci. 14, no. 12: 24087-24096.