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Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China
First Clinical Department of Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Tianjin 300192, China
Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, Tianjin 300134, China
National Institute of Radiological Sciences, Chiba 263-8555, Japan
* Author to whom correspondence should be addressed.
Received: 8 October 2013; in revised form: 19 November 2013 / Accepted: 2 December 2013 / Published: 11 December 2013
Abstract: The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of radiation on the expression of X-linked inhibitor of apoptosis protein (XIAP) were detected by western blotting. The radiation-sensitising effects of ANTP-SmacN7 fusion proteins were observed by a clonogenic assay. The effects of drugs and radiation on tumour cell apoptosis were determined using Annexin V/FITC double staining. Changes in caspase-8, caspase-9 and caspase-3 were detected by western blot before and after ANTP-SmacN7 inhibition of XIAP. The ANTP-SmacN7 fusion protein could enter and accumulate in cells; in vitro XIAP expression of radiation-induced tumour cells was negatively correlated with tumour radiosensitivity. The ANTP-SmacN7 fusion protein promoted tumour cell apoptosis through the activation of caspase3. ANTP-SmacN7 fusion protein may reduce tumour cell radioresistance by inducing caspase3 activation.
Keywords: ANTP-smacN7 fusion protein; apoptosis; radiosensitivity
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Du, L.Q.; Wang, Y.; Xu, C.; Cao, J.; Wang, Q.; Zhao, H.; Fan, F.Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells. Int. J. Mol. Sci. 2013, 14, 24087-24096.
Du LQ, Wang Y, Xu C, Cao J, Wang Q, Zhao H, Fan FY, Wang B, Katsube T, Fan SJ, Liu Q. Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells. International Journal of Molecular Sciences. 2013; 14(12):24087-24096.
Du, Li Q.; Wang, Yan; Xu, Chang; Cao, Jia; Wang, Qin; Zhao, Hui; Fan, Fei Y.; Wang, Bing; Katsube, Takanori; Fan, Sai J.; Liu, Qiang. 2013. "Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells." Int. J. Mol. Sci. 14, no. 12: 24087-24096.