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Int. J. Mol. Sci. 2013, 14(12), 24087-24096; doi:10.3390/ijms141224087

Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells

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1
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
2
Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China
3
First Clinical Department of Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Tianjin 300192, China
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Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, Tianjin 300134, China
5
National Institute of Radiological Sciences, Chiba 263-8555, Japan
*
Author to whom correspondence should be addressed.
Received: 8 October 2013 / Revised: 19 November 2013 / Accepted: 2 December 2013 / Published: 11 December 2013
(This article belongs to the Special Issue Molecular Bases of Cancer Research)
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Abstract

The objective of this study was to investigate the underlying mechanisms behind the radiation-sensitising effects of the antennapedia proteins (ANTP)-smacN7 fusion protein on tumour cells. ANTP-SmacN7 fusion proteins were synthesised, and the ability of this fusion protein to penetrate cells was observed. Effects of radiation on the expression of X-linked inhibitor of apoptosis protein (XIAP) were detected by western blotting. The radiation-sensitising effects of ANTP-SmacN7 fusion proteins were observed by a clonogenic assay. The effects of drugs and radiation on tumour cell apoptosis were determined using Annexin V/FITC double staining. Changes in caspase-8, caspase-9 and caspase-3 were detected by western blot before and after ANTP-SmacN7 inhibition of XIAP. The ANTP-SmacN7 fusion protein could enter and accumulate in cells; in vitro XIAP expression of radiation-induced tumour cells was negatively correlated with tumour radiosensitivity. The ANTP-SmacN7 fusion protein promoted tumour cell apoptosis through the activation of caspase3. ANTP-SmacN7 fusion protein may reduce tumour cell radioresistance by inducing caspase3 activation.
Keywords: ANTP-smacN7 fusion protein; apoptosis; radiosensitivity ANTP-smacN7 fusion protein; apoptosis; radiosensitivity
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Du, L.Q.; Wang, Y.; Xu, C.; Cao, J.; Wang, Q.; Zhao, H.; Fan, F.Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Radiation-Sensitising Effects of Antennapedia Proteins (ANTP)-SmacN7 on Tumour Cells. Int. J. Mol. Sci. 2013, 14, 24087-24096.

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