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Int. J. Mol. Sci. 2013, 14(11), 22449-22461; doi:10.3390/ijms141122449
Article

Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation

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Received: 4 September 2013; in revised form: 25 October 2013 / Accepted: 30 October 2013 / Published: 14 November 2013
(This article belongs to the collection Radiation Toxicity in Cells)
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Abstract: Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
Keywords: radiation; dose-response relationship; DNA damage; comet assay radiation; dose-response relationship; DNA damage; comet assay
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, Y.; Xu, C.; Du, L.Q.; Cao, J.; Liu, J.X.; Su, X.; Zhao, H.; Fan, F.-Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation. Int. J. Mol. Sci. 2013, 14, 22449-22461.

AMA Style

Wang Y, Xu C, Du LQ, Cao J, Liu JX, Su X, Zhao H, Fan F-Y, Wang B, Katsube T, Fan SJ, Liu Q. Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation. International Journal of Molecular Sciences. 2013; 14(11):22449-22461.

Chicago/Turabian Style

Wang, Yan; Xu, Chang; Du, Li Q.; Cao, Jia; Liu, Jian X.; Su, Xu; Zhao, Hui; Fan, Fei-Yue; Wang, Bing; Katsube, Takanori; Fan, Sai J.; Liu, Qiang. 2013. "Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation." Int. J. Mol. Sci. 14, no. 11: 22449-22461.


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