Int. J. Mol. Sci. 2013, 14(11), 22449-22461; doi:10.3390/ijms141122449
Article

Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation

1,2,†email, 1,2,†email, 1,2email, 1,2email, 3,4email, 3,4email, 5email, 1,2email, 6email, 6email, 1,2email and 1,2,* email
1 Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China 2 Tianjin Key Laboratory of Molecular Nuclear Medicine, First Clinical Department of Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Tianjin 300192, China 3 National Institute for Radiological Protection and Nuclear Safety, Chinese Center for Disease Control, Beijing 100088, China 4 Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Beijing 100088, China 5 Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, Tianjin 300134, China 6 National Institute of Radiological Sciences, Chiba 263-8555, Japan These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 4 September 2013; in revised form: 25 October 2013 / Accepted: 30 October 2013 / Published: 14 November 2013
(This article belongs to the Special Issue Radiation Toxicity in Cells)
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Abstract: Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
Keywords: radiation; dose-response relationship; DNA damage; comet assay

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MDPI and ACS Style

Wang, Y.; Xu, C.; Du, L.Q.; Cao, J.; Liu, J.X.; Su, X.; Zhao, H.; Fan, F.-Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation. Int. J. Mol. Sci. 2013, 14, 22449-22461.

AMA Style

Wang Y, Xu C, Du LQ, Cao J, Liu JX, Su X, Zhao H, Fan F-Y, Wang B, Katsube T, Fan SJ, Liu Q. Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation. International Journal of Molecular Sciences. 2013; 14(11):22449-22461.

Chicago/Turabian Style

Wang, Yan; Xu, Chang; Du, Li Q.; Cao, Jia; Liu, Jian X.; Su, Xu; Zhao, Hui; Fan, Fei-Yue; Wang, Bing; Katsube, Takanori; Fan, Sai J.; Liu, Qiang. 2013. "Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation." Int. J. Mol. Sci. 14, no. 11: 22449-22461.

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