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Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China
Tianjin Key Laboratory of Molecular Nuclear Medicine, First Clinical Department of Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Tianjin 300192, China
National Institute for Radiological Protection and Nuclear Safety, Chinese Center for Disease Control, Beijing 100088, China
Medical Emergency Response Center for Nuclear Accidents, Ministry of Health, Beijing 100088, China
Tianjin Key Laboratory of Food and Biotechnology, Tianjin University of Commerce, Tianjin 300134, China
National Institute of Radiological Sciences, Chiba 263-8555, Japan
These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 4 September 2013; in revised form: 25 October 2013 / Accepted: 30 October 2013 / Published: 14 November 2013
Abstract: Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relationship was also found within 72 h after irradiation (p < 0.001). The curves for DNA double-strand breaks and DNA repair in vitro of human lymphocytes presented a nice model, and a smooth, three-dimensional plane model was obtained when the two curves were combined.
Keywords: radiation; dose-response relationship; DNA damage; comet assay
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MDPI and ACS Style
Wang, Y.; Xu, C.; Du, L.Q.; Cao, J.; Liu, J.X.; Su, X.; Zhao, H.; Fan, F.-Y.; Wang, B.; Katsube, T.; Fan, S.J.; Liu, Q. Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation. Int. J. Mol. Sci. 2013, 14, 22449-22461.
Wang Y, Xu C, Du LQ, Cao J, Liu JX, Su X, Zhao H, Fan F-Y, Wang B, Katsube T, Fan SJ, Liu Q. Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation. International Journal of Molecular Sciences. 2013; 14(11):22449-22461.
Wang, Yan; Xu, Chang; Du, Li Q.; Cao, Jia; Liu, Jian X.; Su, Xu; Zhao, Hui; Fan, Fei-Yue; Wang, Bing; Katsube, Takanori; Fan, Sai J.; Liu, Qiang. 2013. "Evaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation." Int. J. Mol. Sci. 14, no. 11: 22449-22461.