Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2013, 14(10), 19618-19635; doi:10.3390/ijms141019618
Article

A Combination of Pre- and Post-Exposure Ascorbic Acid Rescues Mice from Radiation-Induced Lethal Gastrointestinal Damage

1
, 2,* , 1
, 1
, 1
, 3
, 2
 and 1
Received: 26 June 2013; in revised form: 9 September 2013 / Accepted: 13 September 2013 / Published: 27 September 2013
(This article belongs to the collection Radiation Toxicity in Cells)
View Full-Text   |   Download PDF [2141 KB, uploaded 19 June 2014]
Abstract: The development of an effective therapy for radiation-induced gastrointestinal damage is important, because it is currently a major complication of treatment and there are few effective therapies available. Although we have recently demonstrated that pretreatment with ascorbic acid attenuates lethal gastrointestinal damage in irradiated mice, more than half of mice eventually died, thus indicating that better approach was needed. We then investigated a more effective therapy for radiation-induced gastrointestinal damage. Mice receiving abdominal radiation at 13 Gy were orally administered ascorbic acid (250 mg/kg/day) for three days before radiation (pretreatment), one shot of engulfment (250 mg/kg) at 8 h before radiation, or were administered the agent for seven days after radiation (post-treatment). None of the control mice survived the abdominal radiation at 13 Gy due to severe gastrointestinal damage (without bone marrow damage). Neither pretreatment with ascorbic acid (20% survival), engulfment (20%), nor post-treatment (0%) was effective in irradiated mice. However, combination therapy using ascorbic acid, including pretreatment, engulfment and post-treatment, rescued all of the mice from lethal abdominal radiation, and was accompanied by remarkable improvements in the gastrointestinal damage (100% survival). Omitting post-treatment from the combination therapy with ascorbic acid markedly reduced the mouse survival (20% survival), suggesting the importance of post-treatment with ascorbic acid. Combination therapy with ascorbic acid may be a potent therapeutic tool for radiation-induced gastrointestinal damage.
Keywords: ascorbic acid; abdominal radiation; citrulline; radiation-induced gastrointestinal damage; post-irradiation treatment with ascorbic acid ascorbic acid; abdominal radiation; citrulline; radiation-induced gastrointestinal damage; post-irradiation treatment with ascorbic acid
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Ito, Y.; Kinoshita, M.; Yamamoto, T.; Sato, T.; Obara, T.; Saitoh, D.; Seki, S.; Takahashi, Y. A Combination of Pre- and Post-Exposure Ascorbic Acid Rescues Mice from Radiation-Induced Lethal Gastrointestinal Damage. Int. J. Mol. Sci. 2013, 14, 19618-19635.

AMA Style

Ito Y, Kinoshita M, Yamamoto T, Sato T, Obara T, Saitoh D, Seki S, Takahashi Y. A Combination of Pre- and Post-Exposure Ascorbic Acid Rescues Mice from Radiation-Induced Lethal Gastrointestinal Damage. International Journal of Molecular Sciences. 2013; 14(10):19618-19635.

Chicago/Turabian Style

Ito, Yasutoshi; Kinoshita, Manabu; Yamamoto, Tetsuo; Sato, Tomohito; Obara, Takeyuki; Saitoh, Daizoh; Seki, Shuhji; Takahashi, Yukihiro. 2013. "A Combination of Pre- and Post-Exposure Ascorbic Acid Rescues Mice from Radiation-Induced Lethal Gastrointestinal Damage." Int. J. Mol. Sci. 14, no. 10: 19618-19635.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert