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Int. J. Mol. Sci. 2013, 14(11), 21943-21959; doi:10.3390/ijms141121943
Article

Differentiated Epithelial- and Mesenchymal-Like Phenotypes in Subcutaneous Mouse Xenografts Using Diffusion Weighted-Magnetic Resonance Imaging

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Received: 22 July 2013; in revised form: 22 October 2013 / Accepted: 24 October 2013 / Published: 5 November 2013
(This article belongs to the Section Molecular Pathology)
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Abstract: Epithelial-mesenchymal transition (EMT) is important for tumor metastasis. Detection of EMT protein expression and observation of morphological changes are commonly used to identify EMT. Diffusion-weighted magnetic resonance imaging (DW-MRI) and measuring apparent diffusion coefficient (ADC) values are noninvasive techniques for characterizing tumor microenvironments. We investigated the difference in ADC values between epithelial- and mesenchymal-like subcutaneous mouse xenografted tumors using DW-MRI. Epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 cells were generated from tumor suppressive Kruppel-like factor 4 (Klf4)-expressing mesenchymal-like MM189 and BL322 cells. The ADC values of xenografted tumors from epithelial-like MM189 PB-Klf4 and BL322 PB-Klf4 were significantly lower than those from their mesenchymal-like counterparts (p < 0.05 and p < 0.01, respectively). Our results suggested that DW-MRI is a potential tool for observing mesenchymal- or epithelial-like characteristics of subcutaneous xenografted tumors.
Keywords: diffusion-weighted magnetic resonance imaging; epithelial; mesenchymal; apparent diffusion coefficient; mouse xenografts diffusion-weighted magnetic resonance imaging; epithelial; mesenchymal; apparent diffusion coefficient; mouse xenografts
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chen, Y.-W.; Pan, H.-B.; Tseng, H.-H.; Chu, H.-C.; Hung, Y.-T.; Yen, Y.-C.; Chou, C.-P. Differentiated Epithelial- and Mesenchymal-Like Phenotypes in Subcutaneous Mouse Xenografts Using Diffusion Weighted-Magnetic Resonance Imaging. Int. J. Mol. Sci. 2013, 14, 21943-21959.

AMA Style

Chen Y-W, Pan H-B, Tseng H-H, Chu H-C, Hung Y-T, Yen Y-C, Chou C-P. Differentiated Epithelial- and Mesenchymal-Like Phenotypes in Subcutaneous Mouse Xenografts Using Diffusion Weighted-Magnetic Resonance Imaging. International Journal of Molecular Sciences. 2013; 14(11):21943-21959.

Chicago/Turabian Style

Chen, Ya-Wen; Pan, Huay-Ben; Tseng, Hui-Hwa; Chu, Hsiao-Chien; Hung, Yu-Ting; Yen, Yi-Chen; Chou, Chen-Pin. 2013. "Differentiated Epithelial- and Mesenchymal-Like Phenotypes in Subcutaneous Mouse Xenografts Using Diffusion Weighted-Magnetic Resonance Imaging." Int. J. Mol. Sci. 14, no. 11: 21943-21959.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert