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Methanobactin-Mediated One-Step Synthesis of Gold Nanoparticles
Key Laboratory for Food Science & Engineering, Harbin University of Commerce, Harbin 150076, China
State Key Laboratory for Oxo Synthesis & Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou 730000, China
* Author to whom correspondence should be addressed.
Received: 16 September 2013; in revised form: 1 October 2013 / Accepted: 10 October 2013 / Published: 1 November 2013
Abstract: Preparation of gold nanoparticles with a narrow size distribution has enormous importance in nanotechnology. Methanobactin (Mb) is a copper-binding small peptide that appears to function as an agent for copper sequestration and uptake in methanotrophs. Mb can also bind and catalytically reduce Au (III) to Au (0). In this study, we demonstrate a facile Mb-mediated one-step synthetic route to prepare monodispersed gold nanoparticles. Continuous reduction of Au (III) by Mb can be achieved by using hydroquinone as the reducing agent. The gold nanoparticles have been characterized by UV-visible spectroscopy. The formation and the surface plasmon resonance properties of the gold nanoparticles are highly dependent on the ratio of Au (III) to Mb in solution. X-ray photoelectron spectroscopy (XPS), fluorescence spectra and Fourier transform-infrared spectroscopy (FT-IR) spectra suggest that Mb molecules catalytically reduce Au (III) to Au (0) with the concomitant production of gold nanoparticles, and then, Mb statically adsorbed onto the surface of gold nanoparticles to form an Mb-gold nanoparticles assembly. This avoids secondary nucleation. The formed gold nanoparticles have been demonstrated to be monodispersed and uniform by transmission electron microscopy (TEM) images. Analysis of these particles shows an average size of 14.9 nm with a standard deviation of 1.1 nm. The gold nanoparticles are extremely stable and can resist aggregation, even after several months.
Keywords: methanobactin; methanotrophs; gold nanoparticles; hydroquinone; monodisperse
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MDPI and ACS Style
Xin, J.-Y.; Cheng, D.-D.; Zhang, L.-X.; Lin, K.; Fan, H.-C.; Wang, Y.; Xia, C.-G. Methanobactin-Mediated One-Step Synthesis of Gold Nanoparticles. Int. J. Mol. Sci. 2013, 14, 21676-21688.
Xin J-Y, Cheng D-D, Zhang L-X, Lin K, Fan H-C, Wang Y, Xia C-G. Methanobactin-Mediated One-Step Synthesis of Gold Nanoparticles. International Journal of Molecular Sciences. 2013; 14(11):21676-21688.
Xin, Jia-ying; Cheng, Dan-dan; Zhang, Lan-xuan; Lin, Kai; Fan, Hong-chen; Wang, Yan; Xia, Chun-gu. 2013. "Methanobactin-Mediated One-Step Synthesis of Gold Nanoparticles." Int. J. Mol. Sci. 14, no. 11: 21676-21688.