Next Article in Journal
Development and Validation of a Stability-Indicating High Performance Liquid Chromatographic (HPLC) Method for the Determination of Related Substances of Micafungin Sodium in Drug Substances
Next Article in Special Issue
Methanobactin-Mediated One-Step Synthesis of Gold Nanoparticles
Previous Article in Journal
Dietary Polyunsaturated Fatty Acids and Inflammation: The Role of Phospholipid Biosynthesis
Previous Article in Special Issue
Supramolecular Interactions of Terpyridine-Derived Cores of Metallomesogen Precursors
Int. J. Mol. Sci. 2013, 14(10), 21189-21201; doi:10.3390/ijms141021189
Article

Multivalent Protein Assembly Using Monovalent Self-Assembling Building Blocks

,
,
 and *
Laboratory of Chemical Biology and Institute of Complex Molecular Systems, Department of Biomedical Engineering, Eindhoven University of Technology, Den Dolech 2, Eindhoven 5612AZ, The Netherlands
* Author to whom correspondence should be addressed.
Received: 15 August 2013 / Revised: 13 September 2013 / Accepted: 8 October 2013 / Published: 22 October 2013
View Full-Text   |   Download PDF [1608 KB, uploaded 19 June 2014]   |   Browse Figures

Abstract

Discotic molecules, which self-assemble in water into columnar supramolecular polymers, emerged as an alternative platform for the organization of proteins. Here, a monovalent discotic decorated with one single biotin was synthesized to study the self-assembling multivalency of this system in regard to streptavidin. Next to tetravalent streptavidin, monovalent streptavidin was used to study the protein assembly along the supramolecular polymer in detail without the interference of cross-linking. Upon self-assembly of the monovalent biotinylated discotics, multivalent proteins can be assembled along the supramolecular polymer. The concentration of discotics, which influences the length of the final polymers at the same time dictates the amount of assembled proteins.
Keywords: supramolecular polymers; protein-assembly; FRET; multivalency; biotin-streptavidin; self-assembling multivalency supramolecular polymers; protein-assembly; FRET; multivalency; biotin-streptavidin; self-assembling multivalency
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Export to BibTeX |
EndNote


MDPI and ACS Style

Petkau-Milroy, K.; Sonntag, M.H.; Colditz, A.; Brunsveld, L. Multivalent Protein Assembly Using Monovalent Self-Assembling Building Blocks. Int. J. Mol. Sci. 2013, 14, 21189-21201.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert