Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2013, 14(10), 20966-20982; doi:10.3390/ijms141020966
Article

DNA Damage Induced MutS Homologue hMSH4 Acetylation

,
,
,
 and *
Received: 21 August 2013; in revised form: 16 September 2013 / Accepted: 8 October 2013 / Published: 18 October 2013
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [857 KB, uploaded 19 June 2014]   |   Browse Figures
Abstract: Acetylation of non-histone proteins is increasingly recognized as an important post-translational modification for controlling the actions of various cellular processes including DNA repair and damage response. Here, we report that the human MutS homologue hMSH4 undergoes acetylation following DNA damage induced by ionizing radiation (IR). To determine which acetyltransferases are responsible for hMSH4 acetylation in response to DNA damage, potential interactions of hMSH4 with hTip60, hGCN5, and hMof were analyzed. The results of these experiments indicate that only hMof interacts with hMSH4 in a DNA damage-dependent manner. Intriguingly, the interplay between hMSH4 and hMof manipulates the outcomes of nonhomologous end joining (NHEJ)-mediated DNA double strand break (DSB) repair and thereby controls cell survival in response to IR. This study also shows that hMSH4 interacts with HDAC3, by which HDAC3 negatively regulates the levels of hMSH4 acetylation. Interestingly, elevated levels of HDAC3 correlate with increased NHEJ-mediated DSB repair, suggesting that hMSH4 acetylation per se may not directly affect the role of hMSH4 in DSB repair.
Keywords: hMSH4; hMof; acetylation; HDAC3; DSB; ionizing radiation; NHEJ hMSH4; hMof; acetylation; HDAC3; DSB; ionizing radiation; NHEJ
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Chu, Y.-L.; Wu, X.; Xu, J.; Watts, J.L.; Her, C. DNA Damage Induced MutS Homologue hMSH4 Acetylation. Int. J. Mol. Sci. 2013, 14, 20966-20982.

AMA Style

Chu Y-L, Wu X, Xu J, Watts JL, Her C. DNA Damage Induced MutS Homologue hMSH4 Acetylation. International Journal of Molecular Sciences. 2013; 14(10):20966-20982.

Chicago/Turabian Style

Chu, Yen-Lin; Wu, Xiling; Xu, Jing; Watts, Jennifer L.; Her, Chengtao. 2013. "DNA Damage Induced MutS Homologue hMSH4 Acetylation." Int. J. Mol. Sci. 14, no. 10: 20966-20982.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert