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Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat Stress in Chlamydomonas reinhardtii
Department of Plant Medicine, National Pingtung University of Science and Technology, Pingtung 912, Taiwan
Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Taoyuan 320, Taiwan
Institute of Plant and Microbial Biology, Academia Sinica, Taipei 115, Taiwan
Department of Life Science, National Taitung University, Taitung 684, Taiwan
* Author to whom correspondence should be addressed.
Received: 31 August 2013; in revised form: 10 October 2013 / Accepted: 11 October 2013 / Published: 17 October 2013
Abstract: Reactive oxygen species (ROS) produced by plants in adverse environments can cause damage to organelles and trigger cell death. Removal of excess ROS can be achieved through the ascorbate scavenger pathway to prevent plant cell death. The amount of this scavenger can be regulated by ferredoxin (FDX). Chloroplastic FDXs are electron transfer proteins that perform in distributing photosynthetic reducing power. In this study, we demonstrate that overexpression of the endogenous photosynthetic FDX gene, PETF, in Chlamydomonas reinhardtii could raise the level of reduced ascorbate and diminish H2O2 levels under normal growth conditions. Furthermore, the overexpressing PETF transgenic Chlamydomonas lines produced low levels of H2O2 and exhibited protective effects that were observed through decreased chlorophyll degradation and increased cell survival under heat-stress conditions. The findings of this study suggest that overexpression of PETF can increase the efficiency of ROS scavenging in chloroplasts to confer heat tolerance. The roles of PETF in the downregulation of the ROS level offer a method for potentially improving the tolerance of crops against heat stress.
Keywords: Chlamydomonas reinhardtii; ferredoxin; PETF; heat tolerance; ascorbate
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MDPI and ACS Style
Lin, Y.-H.; Pan, K.-Y.; Hung, C.-H.; Huang, H.-E.; Chen, C.-L.; Feng, T.-Y.; Huang, L.-F. Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat Stress in Chlamydomonas reinhardtii. Int. J. Mol. Sci. 2013, 14, 20913-20929.
Lin Y-H, Pan K-Y, Hung C-H, Huang H-E, Chen C-L, Feng T-Y, Huang L-F. Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat Stress in Chlamydomonas reinhardtii. International Journal of Molecular Sciences. 2013; 14(10):20913-20929.
Lin, Yi-Hsien; Pan, Kui-You; Hung, Ching-Hui; Huang, Hsiang-En; Chen, Ching-Lian; Feng, Teng-Yung; Huang, Li-Fen. 2013. "Overexpression of Ferredoxin, PETF, Enhances Tolerance to Heat Stress in Chlamydomonas reinhardtii." Int. J. Mol. Sci. 14, no. 10: 20913-20929.