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Int. J. Mol. Sci. 2013, 14(10), 20157-20170; doi:10.3390/ijms141020157

Microscale Diffusion Measurements and Simulation of a Scaffold with a Permeable Strut

Received: 16 July 2013 / Revised: 6 September 2013 / Accepted: 10 September 2013 / Published: 10 October 2013
(This article belongs to the Special Issue Frontiers of Micro-Spectroscopy in Biological Applications)
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Electrospun nanofibrous structures provide good performance to scaffolds in tissue engineering. We measured the local diffusion coefficients of 3-kDa FITC-dextran in line patterns of electrospun nanofibrous structures fabricated by the direct-write electrospinning (DWES) technique using the fluorescence recovery after photobleaching (FRAP) method. No significant differences were detected between DWES line patterns fabricated with polymer supplied at flow rates of 0.1 and 0.5 mL/h. The oxygen diffusion coefficients of samples were estimated to be ~92%–94% of the oxygen diffusion coefficient in water based on the measured diffusion coefficient of 3-kDa FITC-dextran. We also simulated cell growth and distribution within spatially patterned scaffolds with struts consisting of either oxygen-permeable or non-permeable material. The permeable strut scaffolds exhibited enhanced cell growth. Saturated depths at which cells could grow to confluence were 15% deeper for the permeable strut scaffolds than for the non-permeable strut scaffold.
Keywords: diffusion; electrospinning; fluorescence recovery after photobleaching; scaffold; nanofiber diffusion; electrospinning; fluorescence recovery after photobleaching; scaffold; nanofiber
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Lee, S.Y.; Lee, B.R.; Lee, J.; Kim, S.; Kim, J.K.; Jeong, Y.H.; Jin, S. Microscale Diffusion Measurements and Simulation of a Scaffold with a Permeable Strut. Int. J. Mol. Sci. 2013, 14, 20157-20170.

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