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BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas
Department of Gynecology, the First Affiliated Hospital of China Medical University, Shenyang 110001, China
Department of Biochemistry and Molecular Biology, Institute of Pathology and Pathophysiology, College of Basic Medicine, China Medical University, Shenyang 110001, China
Clinical Cancer Institute, Kanagawa Cancer Center, Yokohama 241-0815, Japan
* Author to whom correspondence should be addressed.
Received: 13 May 2013; in revised form: 5 September 2013 / Accepted: 6 September 2013 / Published: 27 September 2013
Abstract: BTG (B-cell translocation gene) can inhibit cell proliferation, metastasis, and angiogenesis and regulate cell cycle progression and differentiation in a variety of cell types. We aimed to clarify the role of BTG1 in ovarian carcinogenesis and progression. A BTG1-expressing plasmid was transfected into ovarian carcinoma cells and their phenotypes and related proteins were examined. BTG1 mRNA expression was detected in ovarian normal tissue (n = 17), ovarian benign tumors (n = 12), and ovarian carcinoma (n = 64) using real-time RT-PCR. Ectopic BTG1 expression resulted in lower growth rate, high cisplatin sensitivity, G1 arrest, apoptosis, and decreased migration and invasion. Phosphoinositide 3-kinase, protein kinase B, Bcl-xL, survivin, vascular endothelial growth factor, and matrix metalloproteinase-2 mRNA and protein expression was reduced in transfectants as compared to control cells. There was higher expression of BTG1 mRNA in normal tissue than in carcinoma tissue (p = 0.001) and in benign tumors than in carcinoma tissue (p = 0.027). BTG1 mRNA expression in International Federation of Gynecology and Obstetrics (FIGO) stage I/II ovarian carcinomas was higher than that in FIGO stage III/IV ovarian carcinomas (p = 0.038). Altered BTG1 expression might play a role in the pathogenesis and progression of ovarian carcinoma by modulating proliferation, migration, invasion, the cell cycle, and apoptosis.
Keywords: ovarian carcinoma; BTG1; phenotypes; tumorigenesis; progression
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MDPI and ACS Style
Zhao, Y.; Gou, W.-F.; Chen, S.; Takano, Y.; Xiu, Y.-L.; Zheng, H.-C. BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas. Int. J. Mol. Sci. 2013, 14, 19670-19680.
Zhao Y, Gou W-F, Chen S, Takano Y, Xiu Y-L, Zheng H-C. BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas. International Journal of Molecular Sciences. 2013; 14(10):19670-19680.
Zhao, Yang; Gou, Wen-Feng; Chen, Shuo; Takano, Yasuo; Xiu, Yin-Ling; Zheng, Hua-Chuan. 2013. "BTG1 Expression Correlates with the Pathogenesis and Progression of Ovarian Carcinomas." Int. J. Mol. Sci. 14, no. 10: 19670-19680.