Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci
Abstract
:1. Introduction
2. Results
2.1. Analysis of the Bt-mMnSOD Sequence and the Predicted Protein
2.2. Overexpression and Purification of Bt-mMnSOD
2.3. Thermostability of Purified Bt-mMnSOD
2.4. Expression of Bt-mMnSOD during Development
2.5. Effect of Thermal Stresses, Host Shift and Pesticide Treatment on MnSOD Activity
3. Discussion
4. Experimental Section
4.1. Whitefly Cultures and Plants
4.2. cDNA Synthesis and Cloning of the Bt-mMnSOD
4.3. Sequence Analysis
4.4. Overexpression and Purification of Bt-mMnSOD
4.5. Thermostability of Purified Bt-mMnSOD
4.6. Bt-mMnSOD Gene Expression in Different Developmental Stages
4.7. Effect of Thermal Stresses on the MnSOD Activity of the Whitefly
4.8. Effect of Host Shift on the MnSOD Activity of the Whitefly
4.9. Effect of Pesticide Treatment on MnSOD Activity of the Whitefly
5. Conclusions
Acknowledgments
- Conflict of InterestThe authors declare no conflict of interest.
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Species | Protein | Abbreviation | GenBank no. |
---|---|---|---|
Homo sapiens | mitochondrial MnSOD | H. sapiens-mMnSOD | P04179.2 |
Cavia porcellus | mitochondrial MnSOD | C. porcellus-mMnSOD | XM_003466367.1 |
Pongo abelii | mitochondrial MnSOD | P. abelii-mMnSOD | NM_001133563.1 |
Taeniopygia guttata | mitochondrial MnSOD | T. guttata-mMnSOD | DQ214967.1 |
Gallus gallus | mitochondrial MnSOD | G. gallus-mMnSOD | NM_204211.1 |
Melopsittacus undulatus | mitochondrial MnSOD | M. undulatus-mMnSOD | AY241394.1 |
Danio rerio | mitochondrial MnSOD | D. rerio-mMnSOD | NP_956270.1 |
Hemibarbus mylodon | mitochondrial MnSOD | H. mylodon-mMnSOD | ACR23311.1 |
Hypophthalmichthys nobilis | mitochondrial MnSOD | H. nobilis-mMnSOD | ADM26563.1 |
Bemisia tabaci | mitochondrial MnSOD | B. tabaci-mMnSOD | JQ867105 |
Acyrthosiphon pisum | mitochondrial MnSOD | A. pisum-mMnSOD | NM_001246048.1 |
Caenorhabditis elegans | mitochondrial MnSOD | C. elegans-mMnSOD | D12984.1 |
Ancylostoma duodenale | mitochondrial MnSOD | A. duodenale-mMnSOD | FJ465146.1 |
Haliotis discus discus | mitochondrial MnSOD | H. discus-mMnSOD | DQ821491.1 |
Mizuhopecten yessoensis | mitochondrial MnSOD | M. yessoensis-mMnSOD | AB222783.1 |
Callinectes sapidus | mitochondrial MnSOD | C. sapidus-mMnSOD | AF264029.1 |
cytosolic MnSOD | C. sapidus-cMnSOD | AAF74771.1 | |
Scylla paramamosain | mitochondrial MnSOD | S. paramamosain-mMnSOD | FJ605170.2 |
cytosolic MnSOD | S. paramamosain-cMnSOD | ADA63848.1 | |
Portunus trituberculatus | cytosolic MnSOD | P. trituberculatus-cMnSOD | FJ031018.1 |
Fenneropenaeus chinensis | cytosolic MnSOD | F. chinensis-cMnSOD | ACS49842.1 |
Litopenaeus vannamei | cytosolic MnSOD | L. vannamei-cMnSOD | DQ005531.1 |
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Gao, X.-L.; Li, J.-M.; Wang, Y.-L.; Jiu, M.; Yan, G.-H.; Liu, S.-S.; Wang, X.-W. Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci. Int. J. Mol. Sci. 2013, 14, 871-887. https://doi.org/10.3390/ijms14010871
Gao X-L, Li J-M, Wang Y-L, Jiu M, Yan G-H, Liu S-S, Wang X-W. Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci. International Journal of Molecular Sciences. 2013; 14(1):871-887. https://doi.org/10.3390/ijms14010871
Chicago/Turabian StyleGao, Xian-Long, Jun-Min Li, Yong-Liang Wang, Min Jiu, Gen-Hong Yan, Shu-Sheng Liu, and Xiao-Wei Wang. 2013. "Cloning, Expression and Characterization of Mitochondrial Manganese Superoxide Dismutase from the Whitefly, Bemisia tabaci" International Journal of Molecular Sciences 14, no. 1: 871-887. https://doi.org/10.3390/ijms14010871