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Int. J. Mol. Sci. 2013, 14(1), 701-713; doi:10.3390/ijms14010701
Article

The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1

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Received: 26 October 2012; in revised form: 12 December 2012 / Accepted: 12 December 2012 / Published: 4 January 2013
(This article belongs to the Special Issue Abiotic and Biotic Stress Tolerance Mechanisms in Plants)
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Abstract: The voltage-dependent anion channel (VDAC), a highly conserved major mitochondrial outer membrane protein, plays crucial roles in energy metabolism and metabolite transport. However, knowledge about the roles of the VDAC family in plants is limited. In this study, we investigated the expression pattern of VDAC1 in Arabidopsis and found that cold stress promoted the accumulation of VDAC1 transcripts in imbibed seeds and mature plants. Overexpression of VDAC1 reduced tolerance to cold stress in Arabidopsis. Phenotype analysis of VDAC1 T-DNA insertion mutant plants indicated that a vdac1 mutant line had faster germination kinetics under cold treatment and showed enhanced tolerance to freezing. The yeast two-hybrid system revealed that VDAC1 interacts with CBL1, a calcium sensor in plants. Like the vdac1, a cbl1 mutant also exhibited a higher seed germination rate. We conclude that both VDAC1 and CBL1 regulate cold stress responses during seed germination and plant development.
Keywords: Arabidopsis; voltage-dependent anion channel; cold stress; germination; calcium; interaction protein Arabidopsis; voltage-dependent anion channel; cold stress; germination; calcium; interaction protein
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, Z.-Y.; Xu, Z.-S.; He, G.-Y.; Yang, G.-X.; Chen, M.; Li, L.-C.; Ma, Y. The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1. Int. J. Mol. Sci. 2013, 14, 701-713.

AMA Style

Li Z-Y, Xu Z-S, He G-Y, Yang G-X, Chen M, Li L-C, Ma Y. The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1. International Journal of Molecular Sciences. 2013; 14(1):701-713.

Chicago/Turabian Style

Li, Zhi-Yong; Xu, Zhao-Shi; He, Guang-Yuan; Yang, Guang-Xiao; Chen, Ming; Li, Lian-Cheng; Ma, Youzhi. 2013. "The Voltage-Dependent Anion Channel 1 (AtVDAC1) Negatively Regulates Plant Cold Responses during Germination and Seedling Development in Arabidopsis and Interacts with Calcium Sensor CBL1." Int. J. Mol. Sci. 14, no. 1: 701-713.


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