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Wheat Drought-Responsive Grain Proteome Analysis by Linear and Nonlinear 2-DE and MALDI-TOF Mass Spectrometry
College of Life Science, Capital Normal University, Beijing 100048, China
State Agriculture Biotechnology Centre, Western Australian Department of Agriculture and Food, Perth, WA 6150, Australia
These authors contributed equally to this work.
* Authors to whom correspondence should be addressed.
Received: 15 October 2012; in revised form: 13 November 2012 / Accepted: 14 November 2012 / Published: 29 November 2012
Abstract: A comparative proteomic analysis of drought-responsive proteins during grain development of two wheat varieties Kauz (strong resistance to drought stress) and Janz (sensitive to drought stress) was performed by using linear and nonlinear 2-DE and MALDI-TOF mass spectrometry technologies. Results revealed that the nonlinear 2-DE had much higher resolution than the linear 2-DE. A total of 153 differentially expressed protein spots were detected by both 2-DE maps, of which 122 protein spots were identified by MALDI-TOF and MALDI-TOF/TOF mass spectrometry. The identified differential proteins were mainly involved in carbohydrate metabolism (26%), detoxification and defense (23%), and storage proteins (17%). Some key proteins demonstrated significantly different expression patterns between the two varieties. In particular, catalase isozyme 1, WD40 repeat protein, LEA and alpha-amylase inhibitors displayed an upregulated expression pattern in Kauz, whereas they were downregulated or unchanged in Janz. Small and large subunit ADP glucose pyrophosphorylase, ascorbate peroxidase and G beta-like protein were all downregulated under drought stress in Janz, but had no expression changes in Kauz. Sucrose synthase and triticin precursor showed an upregulated expression pattern under water deficits in both varieties, but their upregulation levels were much higher in Kauz than in Janz. These differentially expressed proteins could be related to the biochemical pathways for stronger drought resistance of Kauz.
Keywords: linear and nonlinear 2-DE; MALDI-TOF/TOF MS; comparative proteomics; drought stress; wheat grains
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Table S1. Differentially expressed non-prolamins under control and drought stress conditions in Janz and Kauz by linear 2-DE (pH 3–10,
18 cm) and MALDI mass spectrometry.
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Jiang, S.-S.; Liang, X.-N.; Li, X.; Wang, S.-L.; Lv, D.-W.; Ma, C.-Y.; Li, X.-H.; Ma, W.-J.; Yan, Y.-M. Wheat Drought-Responsive Grain Proteome Analysis by Linear and Nonlinear 2-DE and MALDI-TOF Mass Spectrometry. Int. J. Mol. Sci. 2012, 13, 16065-16083.
Jiang S-S, Liang X-N, Li X, Wang S-L, Lv D-W, Ma C-Y, Li X-H, Ma W-J, Yan Y-M. Wheat Drought-Responsive Grain Proteome Analysis by Linear and Nonlinear 2-DE and MALDI-TOF Mass Spectrometry. International Journal of Molecular Sciences. 2012; 13(12):16065-16083.
Jiang, Shan-Shan; Liang, Xiao-Na; Li, Xin; Wang, Shun-Li; Lv, Dong-Wen; Ma, Chao-Ying; Li, Xiao-Hui; Ma, Wu-Jun; Yan, Yue-Ming. 2012. "Wheat Drought-Responsive Grain Proteome Analysis by Linear and Nonlinear 2-DE and MALDI-TOF Mass Spectrometry." Int. J. Mol. Sci. 13, no. 12: 16065-16083.