Int. J. Mol. Sci. 2013, 14(1), 527-531; doi:10.3390/ijms14010527
Short Note

Isolation and Characterization of Nine Microsatellite Loci for a Parasitoid Wasp, Encarsia smithi (Silvestri) (Hymenoptera: Aphelinidae)

Tea Pest Management Research Team, Department of Tea, National Institute of Vegetable and Tea Science, Kanaya, Shimada, Shizuoka 428-8501, Japan
* Author to whom correspondence should be addressed.
Received: 11 October 2012; in revised form: 18 November 2012 / Accepted: 8 December 2012 / Published: 27 December 2012
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Abstract: The parasitoid wasp Encarsia smithi is an important agent in the classical biological control of two species of invasive spiny whiteflies, Aleurocanthus spiniferus and Aleurocanthus camelliae. To evaluate the performance of parasitism indexed by genetic diversity, a highly polymorphic genetic marker is required. In this report, nine microsatellite loci are described for E. smithi. The microsatellite loci were obtained through the construction of an enriched library and exhibited polymorphisms (2–6 alleles per locus) and high levels of expected heterozygosities (0.203–0.780, average 0.537). Linkage disequilibrium and null alleles were not detected in these microsatellite loci. The isolated microsatellite markers may be useful to estimate the genetic diversity of E. smithi.
Keywords: genetic marker; genetic diversity; microsatellites; spiny whitefly; parasitoid wasp; classical biological control

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MDPI and ACS Style

Uesugi, R.; Sato, Y. Isolation and Characterization of Nine Microsatellite Loci for a Parasitoid Wasp, Encarsia smithi (Silvestri) (Hymenoptera: Aphelinidae). Int. J. Mol. Sci. 2013, 14, 527-531.

AMA Style

Uesugi R, Sato Y. Isolation and Characterization of Nine Microsatellite Loci for a Parasitoid Wasp, Encarsia smithi (Silvestri) (Hymenoptera: Aphelinidae). International Journal of Molecular Sciences. 2013; 14(1):527-531.

Chicago/Turabian Style

Uesugi, Ryuji; Sato, Yasushi. 2013. "Isolation and Characterization of Nine Microsatellite Loci for a Parasitoid Wasp, Encarsia smithi (Silvestri) (Hymenoptera: Aphelinidae)." Int. J. Mol. Sci. 14, no. 1: 527-531.

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