Int. J. Mol. Sci. 2013, 14(1), 1152-1163; doi:10.3390/ijms14011152
Article

Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins

1 Department of Crystallography and Structural Biology, Institute of Physical-Chemistry "Rocasolano", CSIC, Serrano 119, Madrid 28006, Spain 2 Institute of Molecular and Cellular Biology of Rosario (National University of Rosario and CONICET), Suipacha 531, S2002LRK-Rosario, Argentina
* Author to whom correspondence should be addressed.
Received: 1 November 2012; in revised form: 14 November 2012 / Accepted: 20 November 2012 / Published: 9 January 2013
(This article belongs to the Special Issue Flavins)
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Abstract: Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50’s loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as “short-chain” with the firmicutes flavodoxins and the “long-chain” with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase.
Keywords: flavodoxin; nitrogen fixation; crystal structure; phylogenetic analysis

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MDPI and ACS Style

Pérez-Dorado, I.; Bortolotti, A.; Cortez, N.; Hermoso, J.A. Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins. Int. J. Mol. Sci. 2013, 14, 1152-1163.

AMA Style

Pérez-Dorado I, Bortolotti A, Cortez N, Hermoso JA. Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins. International Journal of Molecular Sciences. 2013; 14(1):1152-1163.

Chicago/Turabian Style

Pérez-Dorado, Inmaculada; Bortolotti, Ana; Cortez, Néstor; Hermoso, Juan A. 2013. "Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins." Int. J. Mol. Sci. 14, no. 1: 1152-1163.

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