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Int. J. Mol. Sci. 2013, 14(1), 1152-1163; doi:10.3390/ijms14011152
Article

Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins

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Received: 1 November 2012; in revised form: 14 November 2012 / Accepted: 20 November 2012 / Published: 9 January 2013
(This article belongs to the Special Issue Flavins)
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Abstract: Analysis of the crystal structure of NifF from Rhodobacter capsulatus and its homologues reported so far reflects the existence of unique structural features in nif flavodoxins: a leucine at the re face of the isoalloxazine, an eight-residue insertion at the C-terminus of the 50’s loop and a remarkable difference in the electrostatic potential surface with respect to non-nif flavodoxins. A phylogenetic study on 64 sequences from 52 bacterial species revealed four clusters, including different functional prototypes, correlating the previously defined as “short-chain” with the firmicutes flavodoxins and the “long-chain” with gram-negative species. The comparison of Rhodobacter NifF structure with other bacterial flavodoxin prototypes discloses the concurrence of specific features of these functional electron donors to nitrogenase.
Keywords: flavodoxin; nitrogen fixation; crystal structure; phylogenetic analysis flavodoxin; nitrogen fixation; crystal structure; phylogenetic analysis
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Pérez-Dorado, I.; Bortolotti, A.; Cortez, N.; Hermoso, J.A. Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins. Int. J. Mol. Sci. 2013, 14, 1152-1163.

AMA Style

Pérez-Dorado I, Bortolotti A, Cortez N, Hermoso JA. Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins. International Journal of Molecular Sciences. 2013; 14(1):1152-1163.

Chicago/Turabian Style

Pérez-Dorado, Inmaculada; Bortolotti, Ana; Cortez, Néstor; Hermoso, Juan A. 2013. "Structural and Phylogenetic Analysis of Rhodobacter capsulatus NifF: Uncovering General Features of Nitrogen-fixation (nif)-Flavodoxins." Int. J. Mol. Sci. 14, no. 1: 1152-1163.


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