Abstract: Influence of gravity forces on the regulation of protein expression by healthy and malignant thyroid cells was studied with the aim to identify protein interactions. Western blot analyses of a limited number of proteins suggested a time-dependent regulation of protein expression by simulated microgravity. After applying free flow isoelectric focusing and mass spectrometry to search for differently expressed proteins by thyroid cells exposed to simulated microgravity for three days, a considerable number of candidates for gravi-sensitive proteins were detected. In order to show how proteins sensitive to microgravity could directly influence other proteins, we investigated all polypeptide chains identified with Mascot scores above 100, looking for groups of interacting proteins. Hence, UniProtKB entry numbers of all detected proteins were entered into the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) and processed. The program indicated that we had detected various groups of interacting proteins in each of the three cell lines studied. The major groups of interacting proteins play a role in pathways of carbohydrate and protein metabolism, regulation of cell growth and cell membrane structuring. Analyzing these groups, networks of interaction could be established which show how a punctual influence of simulated microgravity may propagate via various members of interaction chains.
Keywords: thyroid cancer cell line FTC-133; thyroid cancer cell line CGTH W-1; normal thyrocyte cells HTU-5; Random Positioning Machine (RPM); STRING network analysis
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Pietsch, J.; Riwaldt, S.; Bauer, J.; Sickmann, A.; Weber, G.; Grosse, J.; Infanger, M.; Eilles, C.; Grimm, D. Interaction of Proteins Identified in Human Thyroid Cells. Int. J. Mol. Sci. 2013, 14, 1164-1178.
Pietsch J, Riwaldt S, Bauer J, Sickmann A, Weber G, Grosse J, Infanger M, Eilles C, Grimm D. Interaction of Proteins Identified in Human Thyroid Cells. International Journal of Molecular Sciences. 2013; 14(1):1164-1178.
Pietsch, Jessica; Riwaldt, Stefan; Bauer, Johann; Sickmann, Albert; Weber, Gerhard; Grosse, Jirka; Infanger, Manfred; Eilles, Christoph; Grimm, Daniela. 2013. "Interaction of Proteins Identified in Human Thyroid Cells." Int. J. Mol. Sci. 14, no. 1: 1164-1178.