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Int. J. Mol. Sci. 2012, 13(9), 11870-11880; doi:10.3390/ijms130911870
Article

Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme

1,* , 1
, 2
 and 1,3
Received: 16 July 2012; in revised form: 27 August 2012 / Accepted: 3 September 2012 / Published: 19 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Abstract: Modified polystyrene with Pt(IV)–azomethine (APS–Sch–Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS–Sch–Pt–GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)–azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS–Sch–Pt–GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.
Keywords: Pt(IV)–azomethine; Polystyrene; glucose oxidase enzyme; two optimum pH Pt(IV)–azomethine; Polystyrene; glucose oxidase enzyme; two optimum pH
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Sari, N.; Antepli, E.; Nartop, D.; Yetim, N.K. Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme. Int. J. Mol. Sci. 2012, 13, 11870-11880.

AMA Style

Sari N, Antepli E, Nartop D, Yetim NK. Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme. International Journal of Molecular Sciences. 2012; 13(9):11870-11880.

Chicago/Turabian Style

Sari, Nurşen; Antepli, Esin; Nartop, Dilek; Yetim, Nurdan Kurnaz. 2012. "Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme." Int. J. Mol. Sci. 13, no. 9: 11870-11880.



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