Next Article in Journal
Encapsulation-Induced Stress Helps Saccharomyces cerevisiae Resist Convertible Lignocellulose Derived Inhibitors
Next Article in Special Issue
Purification and Characterization of a Ginsenoside Rb1-Hydrolyzing β-Glucosidase from Aspergillus niger KCCM 11239
Previous Article in Journal
Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae
Previous Article in Special Issue
High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435
Int. J. Mol. Sci. 2012, 13(9), 11870-11880; doi:10.3390/ijms130911870
Article

Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme

1,* , 1
,
2
 and
1,3
Received: 16 July 2012 / Revised: 27 August 2012 / Accepted: 3 September 2012 / Published: 19 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
View Full-Text   |   Download PDF [379 KB, 19 June 2014; original version 19 June 2014]   |   Browse Figures

Abstract

Modified polystyrene with Pt(IV)–azomethine (APS–Sch–Pt) was synthesized by means of condensation and demonstrated to be a promising enzyme support by studying the enzymatic properties of glucose oxidase enzyme (GOx) immobilized on it. The characteristics of the immobilized glucose oxidase (APS–Sch–Pt–GOx) enzyme showed two optimum pH values that were pH = 4.0 and pH = 7. The insertion of stable Pt(IV)–azomethine spacers between the polystyrene backbone and the immobilized GOx, (APS–Sch–Pt–GOx), increases the enzymes’ activity and improves their affinity towards the substrate even at pH = 4. The influence of temperature, reusability and storage capacity on the free and immobilized glucose oxidase enzyme was investigated. The storage stability of the immobilized glucose oxidase was shown to be eleven months in dry conditions at +4 °C.
Keywords: Pt(IV)–azomethine; Polystyrene; glucose oxidase enzyme; two optimum pH Pt(IV)–azomethine; Polystyrene; glucose oxidase enzyme; two optimum pH
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote
MDPI and ACS Style

Sari, N.; Antepli, E.; Nartop, D.; Yetim, N.K. Polystyrene Attached Pt(IV)–Azomethine, Synthesis and Immobilization of Glucose Oxidase Enzyme. Int. J. Mol. Sci. 2012, 13, 11870-11880.

View more citation formats

Related Articles

Article Metrics

Comments

Citing Articles

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert