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Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae
Department of Plant Biology and Ecology, CITE-2, University of Almería, Carretera de Sacramento s/n, E-04120 La Cañada de San Urbano, Almería, Spain
Desertification Research Center (CIDE, CSIC-UV-GV), Carretera de Moncada-Náquera Km 4.5, E-46113 Moncada, Valencia, Spain
* Author to whom correspondence should be addressed.
Received: 8 August 2012; in revised form: 31 August 2012 / Accepted: 5 September 2012 / Published: 19 September 2012
Abstract: Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte Lepidium subulatum to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci including di- and tri-nucleotide repeats were characterized in L. subulatum. They amplified a total of 80 alleles (2–12 alleles per locus) in a sample of 35 individuals of L. subulatum, showing relatively high levels of genetic diversity, HO = 0.645, HE = 0.627. Cross-species transferability of all 12 loci was successful for the Iberian endemics Lepidium cardamines, Lepidium stylatum, and the widespread, Lepidium graminifolium and one species each of two related genera, Cardaria draba and Coronopus didymus. These microsatellite primers will be useful to investigate genetic diversity, population structure and to address conservation genetics in species of Lepidium.
Keywords: Brassicaceae; conservation genetics; genetic diversity; gypsophile; next-generation sequencing; SSR
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Martínez-Nieto, M.I.; Encarna Merlo, M.; Mota, J.F.; Salmerón-Sánchez, E.; Segarra-Moragues, J.G. Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae. Int. J. Mol. Sci. 2012, 13, 11861-11869.
Martínez-Nieto MI, Encarna Merlo M, Mota JF, Salmerón-Sánchez E, Segarra-Moragues JG. Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae. International Journal of Molecular Sciences. 2012; 13(9):11861-11869.
Martínez-Nieto, María Isabel; Encarna Merlo, María; Mota, Juan Francisco; Salmerón-Sánchez, Esteban; Segarra-Moragues, José Gabriel. 2012. "Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae." Int. J. Mol. Sci. 13, no. 9: 11861-11869.