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• Martínez-Nieto, M
• Encarna Merlo, M
• Mota, J
• Salmerón-Sánchez, E
• Segarra-Moragues, J
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• Martínez-Nieto, M
• Encarna Merlo, M
• Mota, J
• Salmerón-Sánchez, E
• Segarra-Moragues, J
• [+] on PubMed
• Martínez-Nieto, M
• Encarna Merlo, M
• Mota, J
• Salmerón-Sánchez, E
• Segarra-Moragues, J

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Int. J. Mol. Sci. 2012, 13(9), 11861-11869; doi:10.3390/ijms130911861

Article

Microsatellite Loci in the Gypsophyte Lepidium subulatum (Brassicaceae), and Transferability to Other Lepidieae

María Isabel Martínez-Nieto ^1,* , María Encarna Merlo ¹ , Juan Francisco Mota ¹ , Esteban Salmerón-Sánchez ¹  and José Gabriel Segarra-Moragues ²

¹ Department of Plant Biology and Ecology, CITE-2, University of Almería, Carretera de Sacramento s/n, E-04120 La Cañada de San Urbano, Almería, Spain ² Desertification Research Center (CIDE, CSIC-UV-GV), Carretera de Moncada-Náquera Km 4.5, E-46113 Moncada, Valencia, Spain

* Author to whom correspondence should be addressed.

Received: 8 August 2012; in revised form: 31 August 2012 / Accepted: 5 September 2012 / Published: 19 September 2012

(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)

Download PDF Full-Text [1229 KB, uploaded 19 September 2012 15:19 CEST]

Abstract: Polymorphic microsatellite markers were developed for the Ibero-North African, strict gypsophyte Lepidium subulatum to unravel the effects of habitat fragmentation in levels of genetic diversity, genetic structure and gene flow among its populations. Using 454 pyrosequencing 12 microsatellite loci including di- and tri-nucleotide repeats were characterized in L. subulatum. They amplified a total of 80 alleles (2–12 alleles per locus) in a sample of 35 individuals of L. subulatum, showing relatively high levels of genetic diversity, H_O = 0.645, H_E = 0.627. Cross-species transferability of all 12 loci was successful for the Iberian endemics Lepidium cardamines, Lepidium stylatum, and the widespread, Lepidium graminifolium and one species each of two related genera, Cardaria draba and Coronopus didymus. These microsatellite primers will be useful to investigate genetic diversity, population structure and to address conservation genetics in species of Lepidium.

Keywords: Brassicaceae; conservation genetics; genetic diversity; gypsophile; next-generation sequencing; SSR

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