Int. J. Mol. Sci. 2012, 13(9), 11694-11704; doi:10.3390/ijms130911694
Article

High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

Received: 23 July 2012; in revised form: 3 September 2012 / Accepted: 3 September 2012 / Published: 17 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme® RMIM (Rhizomucor miehei) and Novozym® 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio (1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme® RMIM and Novozym® 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym® 435 proves to be a more efficient biocatalyst than Lipozyme® RMIM.
Keywords: optimization; wax esters; lipase; esterification; Rhizomucor miehei; Candida antarctica
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MDPI and ACS Style

Kuo, C.-H.; Chen, H.-H.; Chen, J.-H.; Liu, Y.-C.; Shieh, C.-J. High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435. Int. J. Mol. Sci. 2012, 13, 11694-11704.

AMA Style

Kuo C-H, Chen H-H, Chen J-H, Liu Y-C, Shieh C-J. High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435. International Journal of Molecular Sciences. 2012; 13(9):11694-11704.

Chicago/Turabian Style

Kuo, Chia-Hung; Chen, Hsin-Hung; Chen, Jiann-Hwa; Liu, Yung-Chuan; Shieh, Chwen-Jen. 2012. "High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435." Int. J. Mol. Sci. 13, no. 9: 11694-11704.

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