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Int. J. Mol. Sci. 2012, 13(9), 11694-11704; doi:10.3390/ijms130911694
Article

High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435

1
,
2
,
3
,
4
 and
1,*
1 Biotechnology Center, National Chung Hsing University, 250 Kuo-kuang Road, Taichung 402, Taiwan 2 Department and Graduate Program of Bioindustry Technology, Dayeh University, 168 University Road, Chang-Hwa, 515, Taiwan 3 Graduate Institute of Molecular Biology, National Chung Hsing University, 250 Kuo-kuang Road, Taichung, 402, Taiwan 4 Department of Chemical Engineering, National Chung Hsing University, 250 Kuo-kuang Road, Taichung, 402, Taiwan
* Author to whom correspondence should be addressed.
Received: 23 July 2012 / Revised: 3 September 2012 / Accepted: 3 September 2012 / Published: 17 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Abstract

Wax esters are long-chain esters that have been widely applied in premium lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two commercial immobilized lipases, i.e., Lipozyme® RMIM (Rhizomucor miehei) and Novozym® 435 (Candida antarctica). Response surface methodology (RSM) and 5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio (1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme® RMIM and Novozym® 435, respectively. The optimum conditions for synthesis of cetyl octanoate by both lipases are established and compared. Novozym® 435 proves to be a more efficient biocatalyst than Lipozyme® RMIM.
Keywords: optimization; wax esters; lipase; esterification; Rhizomucor miehei; Candida antarctica optimization; wax esters; lipase; esterification; Rhizomucor miehei; Candida antarctica
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Kuo, C.-H.; Chen, H.-H.; Chen, J.-H.; Liu, Y.-C.; Shieh, C.-J. High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435. Int. J. Mol. Sci. 2012, 13, 11694-11704.

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