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Int. J. Mol. Sci. 2012, 13(9), 12140-12152; doi:10.3390/ijms130912140
Article

Purification and Characterization of a Ginsenoside Rb1-Hydrolyzing β-Glucosidase from Aspergillus niger KCCM 11239

1
, 1
, 2
 and 1,2,*
Received: 13 July 2012; in revised form: 24 August 2012 / Accepted: 7 September 2012 / Published: 24 September 2012
(This article belongs to the Special Issue Enzyme Optimization and Immobilization)
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Abstract: Rb1-hydrolyzing β-glucosidase from Aspergillus niger KCCM 11239 was studied to develop a bioconversion process for minor ginsenosides. The specific activity of the purified enzyme was 46.5 times greater than that of the crude enzyme. The molecular weight of the native enzyme was estimated to be approximately 123 kDa. The optimal pH of the purified enzyme was pH 4.0, and the enzyme proved highly stable over a pH range of 5.0–10.0. The optimal temperature was 70 °C, and the enzyme became unstable at temperatures above 60 °C. The enzyme was inhibited by Cu2+, Mg2+, Co2+, and acetic acid (10 mM). In the specificity tests, the enzyme was found to be active against ginsenoside Rb1, but showed very low levels of activity against Rb2, Rc, Rd, Re, and Rg1. The enzyme hydrolyzed the 20-C,β-(1→6)-glucoside of ginsenoside Rb1 to generate ginsenoside Rd and Rg3, and hydrolyzed 3-C,β-(1→2)-glucoside to generate F2. The properties of the enzyme indicate that it could be a useful tool in biotransformation applications in the ginseng industry, as well as in the development of novel drug compounds.
Keywords: β-glucosidase; Aspergillus niger KCCM 11239; ginsenoside Rb1; ginsenoside Rd; ginsenoside F2 β-glucosidase; Aspergillus niger KCCM 11239; ginsenoside Rb1; ginsenoside Rd; ginsenoside F2
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chang, K.H.; Jo, M.N.; Kim, K.-T.; Paik, H.-D. Purification and Characterization of a Ginsenoside Rb1-Hydrolyzing β-Glucosidase from Aspergillus niger KCCM 11239. Int. J. Mol. Sci. 2012, 13, 12140-12152.

AMA Style

Chang KH, Jo MN, Kim K-T, Paik H-D. Purification and Characterization of a Ginsenoside Rb1-Hydrolyzing β-Glucosidase from Aspergillus niger KCCM 11239. International Journal of Molecular Sciences. 2012; 13(9):12140-12152.

Chicago/Turabian Style

Chang, Kyung Hoon; Jo, Mi Na; Kim, Kee-Tae; Paik, Hyun-Dong. 2012. "Purification and Characterization of a Ginsenoside Rb1-Hydrolyzing β-Glucosidase from Aspergillus niger KCCM 11239." Int. J. Mol. Sci. 13, no. 9: 12140-12152.



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