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Int. J. Mol. Sci. 2012, 13(9), 11275-11287; doi:10.3390/ijms130911275
Article

Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice

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1 College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China 2 Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China 3 Institute of Plant Protection, Liaoning Academy of Agricultural Sciences, Shenyang 110161, China
* Author to whom correspondence should be addressed.
Received: 20 July 2012 / Revised: 10 August 2012 / Accepted: 6 September 2012 / Published: 10 September 2012
(This article belongs to the Special Issue Plant-Derived Pharmaceuticals by Molecular Farming 2012)
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Abstract

Ustiloxins are cyclopeptide mycotoxins produced by the pathogenic fungus Villosiclava virens of rice false smut. Ustiloxins A and B as two main mycotoxins were determined conveniently by LC-ESI-MS in the water extract from rice false smut balls which were mostly composed of the chlamydospores and mycelia of the pathogen. Both ustiloxins A and B in the water extract were also quantitatively analyzed by HPLC. This is the first report on the determination and analysis of ustiloxins A and B simultaneously by LC-ESI-MS and HPLC in false smut balls of rice.
Keywords: Villosiclava virens; ustiloxins A and B; false smut balls of rice; determination and analysis; LC-ESI-MS; HPLC Villosiclava virens; ustiloxins A and B; false smut balls of rice; determination and analysis; LC-ESI-MS; HPLC
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Shan, T.; Sun, W.; Liu, H.; Gao, S.; Lu, S.; Wang, M.; Sun, W.; Chen, Z.; Wang, S.; Zhou, L. Determination and Analysis of Ustiloxins A and B by LC-ESI-MS and HPLC in False Smut Balls of Rice. Int. J. Mol. Sci. 2012, 13, 11275-11287.

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