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Int. J. Mol. Sci. 2012, 13(9), 11260-11274; doi:10.3390/ijms130911260

Quantification and Purification of Mangiferin from Chinese Mango (Mangifera indica L.) Cultivars and Its Protective Effect on Human Umbilical Vein Endothelial Cells under H2O2-induced Stress

1
Laboratory of Fruit Quality Biology/The State Agriculture Ministry Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
2
College of Pharmaceutical Sciences, Zhejiang University, Zijingang Campus, Hangzhou 310058, China
3
College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong, China
4
Guangxi Subtropical Crop Research Institute, Nanning 530001, Guangxi, China
*
Author to whom correspondence should be addressed.
Received: 1 August 2012 / Revised: 28 August 2012 / Accepted: 3 September 2012 / Published: 10 September 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Mangiferin is a natural xanthonoid with various biological activities. Quantification of mangiferin in fruit peel, pulp, and seed kernel was carried out in 11 Chinese mango (Mangifera indica L.) cultivars. The highest mangiferin content was found in the peel of Lvpimang (LPM) fruit (7.49 mg/g DW). Efficient purification of mangiferin from mango fruit peel was then established for the first time by combination of macroporous HPD100 resin chromatography with optimized high-speed counter-current chromatography (HSCCC). Purified mangiferin was identified by both HPLC and LC-MS, and it showed higher DPPH free-radical scavenging capacities and ferric reducing ability of plasma (FRAP) than by l-ascorbic acid (Vc) or Trolox. In addition, it showed significant protective effects on human umbilical vein endothelial cells (HUVEC) under H2O2-induced stress. Cells treated with mangiferin resulted in significant enhanced cell survival under of H2O2 stress. Therefore, mangiferin from mango fruit provides a promising perspective for the prevention of oxidative stress-associated diseases. View Full-Text
Keywords: Mangifera indica; mangiferin; purification; antioxidant; HUVEC; protective effect Mangifera indica; mangiferin; purification; antioxidant; HUVEC; protective effect
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Luo, F.; Lv, Q.; Zhao, Y.; Hu, G.; Huang, G.; Zhang, J.; Sun, C.; Li, X.; Chen, K. Quantification and Purification of Mangiferin from Chinese Mango (Mangifera indica L.) Cultivars and Its Protective Effect on Human Umbilical Vein Endothelial Cells under H2O2-induced Stress. Int. J. Mol. Sci. 2012, 13, 11260-11274.

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