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Int. J. Mol. Sci. 2012, 13(9), 11188-11193; doi:10.3390/ijms130911188
Short Note

Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome

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Received: 6 August 2012; in revised form: 3 September 2012 / Accepted: 3 September 2012 / Published: 7 September 2012
(This article belongs to the Section Molecular Pathology)
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Abstract: In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (HE) and observed heterozygosity (HO) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.
Keywords: microsatellite markers; Phaseolus vulgaris L.; cross-species transferability; genetic diversity microsatellite markers; Phaseolus vulgaris L.; cross-species transferability; genetic diversity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Wang, A.; Ding, Y.; Hu, Z.; Lin, C.; Wang, S.; Wang, B.; Zhang, H.; Zhou, G. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. Int. J. Mol. Sci. 2012, 13, 11188-11193.

AMA Style

Wang A, Ding Y, Hu Z, Lin C, Wang S, Wang B, Zhang H, Zhou G. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. International Journal of Molecular Sciences. 2012; 13(9):11188-11193.

Chicago/Turabian Style

Wang, Aihua; Ding, Yi; Hu, Zhenhua; Lin, Chufa; Wang, Shuzhen; Wang, Bingcai; Zhang, Hongyuan; Zhou, Guolin. 2012. "Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome." Int. J. Mol. Sci. 13, no. 9: 11188-11193.


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