Int. J. Mol. Sci. 2012, 13(9), 11188-11193; doi:10.3390/ijms130911188
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Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome

1 Hubei Provincial Engineering Research Center of Vegetable Seeds and Seedlings, Wuhan Vegetable Research Institute, Wuhan Academy of Agricultural Science & Technology, Wuhan 430345, China 2 State Key Laboratory of Hybrid Rice, Department of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China
* Author to whom correspondence should be addressed.
Received: 6 August 2012; in revised form: 3 September 2012 / Accepted: 3 September 2012 / Published: 7 September 2012
(This article belongs to the Section Molecular Diagnostics)
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Abstract: In this study, 13 polymorphic microsatellite markers were isolated from the Phaseolus vulgaris L. (common bean) by using the Fast Isolation by AFLP of Sequence COntaining Repeats (FIASCO) protocol. These markers revealed two to seven alleles, with an average of 3.64 alleles per locus. The polymorphic information content (PIC) values ranged from 0.055 to 0.721 over 13 loci, with a mean value of 0.492, and 7 loci having PIC greater than 0.5. The expected heterozygosity (HE) and observed heterozygosity (HO) levels ranged from 0.057 to 0.814 and from 0.026 to 0.531, respectively. Cross-species amplification of the 13 prime pairs was performed in its related specie of Vigna unguiculata L. Seven out of all these markers showed cross-species transferability. These markers will be useful for future genetic diversity and population genetics studies for this agricultural specie and its related species.
Keywords: microsatellite markers; Phaseolus vulgaris L.; cross-species transferability; genetic diversity

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MDPI and ACS Style

Wang, A.; Ding, Y.; Hu, Z.; Lin, C.; Wang, S.; Wang, B.; Zhang, H.; Zhou, G. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. Int. J. Mol. Sci. 2012, 13, 11188-11193.

AMA Style

Wang A, Ding Y, Hu Z, Lin C, Wang S, Wang B, Zhang H, Zhou G. Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome. International Journal of Molecular Sciences. 2012; 13(9):11188-11193.

Chicago/Turabian Style

Wang, Aihua; Ding, Yi; Hu, Zhenhua; Lin, Chufa; Wang, Shuzhen; Wang, Bingcai; Zhang, Hongyuan; Zhou, Guolin. 2012. "Isolation and Characterization of 13 New Polymorphic Microsatellite Markers in the Phaseolus vulgaris L. (Common Bean) Genome." Int. J. Mol. Sci. 13, no. 9: 11188-11193.

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