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Int. J. Mol. Sci. 2012, 13(9), 11165-11172; doi:10.3390/ijms130911165
Short Note

Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance

2,*  and 1,*
1 Department of Botany, School of Life Sciences, Yunnan University, Kunming 650091, China 2 Research Institute of Resource Insects, Chinese Academy of Forest (CAF), Kunming 650224, China
* Authors to whom correspondence should be addressed.
Received: 7 August 2012 / Revised: 28 August 2012 / Accepted: 2 September 2012 / Published: 7 September 2012
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Cephalotaxus oliveri is a scarce medicinal conifer endemic to the south central region of China and Vietnam. A small fragmented population presently exists due to anthropogenic disturbance. C. oliveri has been used for its alkaloids harringtonine and homoharringtonine, which are effective against leucocythemia and lymphadenosarcoma. Monoecious plants have been detected in nature, although they were understood to be dioecious. In order to study the mating system, population genetics and the genetic effects of habitat fragmentation on C. oliveri, 15 polymorphic and 12 monomorphic microsatellite loci were developed for C. oliveri by using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol. The polymorphisms were assessed in 96 individuals from three natural populations (32 individuals per population). The number of alleles per locus ranged from two to 33, the observed and expected heterozygosity per locus ranged from 0.000 to 1.000 and from 0.000 to 0.923, respectively. These loci would facilitate a comprehensive understanding of the genetic dynamics on C. oliveri, which will be useful for establishing effective conservation strategies for this species.
Keywords: Cephalotaxus oliveri; FIASCO; microsatellite loci; PCR amplification Cephalotaxus oliveri; FIASCO; microsatellite loci; PCR amplification
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Miao, Y.; Lang, X.; Li, S.; Su, J.; Wang, Y. Characterization of 15 Polymorphic Microsatellite Loci for Cephalotaxus oliveri (Cephalotaxaceae), a Conifer of Medicinal Importance. Int. J. Mol. Sci. 2012, 13, 11165-11172.

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